Shokri F, Mageed R A, Jefferis R
Department of Immunology, Medical School, University of Birmingham, Edgbaston.
Br J Rheumatol. 1993 Oct;32(10):862-9. doi: 10.1093/rheumatology/32.10.862.
A sensitive ELISA for the quantitation of RF associated cross-reactive idiotypes (CRI) has been developed. CRI bearing IgM was captured by immobilized F(ab')2 fragments of monoclonal anti-CRI antibodies and revealed with isotype specific conjugates. Factors affecting sensitivity, specificity and reproducibility of the assay were assessed. The sensitivity limit was less than 10 ng/ml and the linear assay range for the CRI standard curve was from 80 to 500 ng/ml. Using this ELISA system we have quantitated serum levels of the CRI in different groups of patients with RA, early synovitis and normal individuals. Our results indicate a significant increase in the levels of the CRI in the patient groups compared to the normal subjects tested, and suggest involvement of a diverse repertoire of Ig variable region germline genes and/or somatic mutation in production of polyclonal RF in RA.
已开发出一种用于定量类风湿因子相关交叉反应性独特型（CRI）的灵敏酶联免疫吸附测定法（ELISA）。携带CRI的IgM通过固定化的单克隆抗CRI抗体的F(ab')2片段捕获，并用同型特异性偶联物显示。评估了影响该测定法灵敏度、特异性和可重复性的因素。灵敏度极限小于10 ng/ml，CRI标准曲线的线性测定范围为80至500 ng/ml。使用该ELISA系统，我们已对不同组类风湿性关节炎（RA）患者、早期滑膜炎患者和正常个体的血清CRI水平进行了定量。我们的结果表明，与所检测的正常受试者相比，患者组中CRI水平显著升高，并提示在RA中多克隆类风湿因子的产生涉及多种Ig可变区种系基因和/或体细胞突变。
