Adams G K, Lichtenstein L
J Immunol. 1979 Feb;122(2):555-62.
Exposure of sensitized guinea pig tracheal rings or human bronchial strips to specific antigen in vitro resulted in a rapidly developing, prolonged contraction that was resistant to washing. Treatment of the tissue with diphenhydramine, a histamine H1 antagonist, before antigen delayed the onset and decreased the amplitude of the initial phase of the contraction but did not reduce the duration. Diphenhydramine treatment after development of the contraction did not relax the airway tissue. Antigen-induced histamine release from guinea pig trachea and from human bronchus was complete within the initial 15% of the duration of the contraction. Treatment of sensitized airway tissue with FPL 55712, a SRS-A antagonist, before antigen selectively inhibited the prolonged phase of the response. FPL 55712 administration after the development of antigen-induced contraction resulted in relaxation. These data suggest that both histamine and SRS-A are involved in the response of sensitized guinea pig and human airway tissue to antigen, with histamine mediating the early phase of the contraction and SRS-A primarily mediating the protracted phase.
在体外将致敏豚鼠气管环或人支气管条暴露于特异性抗原会导致迅速发生且持续时间较长的收缩，这种收缩对冲洗具有抗性。在用组胺H1拮抗剂苯海拉明处理组织后再接触抗原，会延迟收缩起始并降低收缩初始阶段的幅度，但不会缩短持续时间。在收缩发生后用苯海拉明处理并不能使气道组织松弛。抗原诱导的组胺从豚鼠气管和人支气管中的释放，在收缩持续时间的最初15%内完成。在用慢反应物质A（SRS - A）拮抗剂FPL 55712处理致敏气道组织后再接触抗原，可选择性抑制反应的延长阶段。在抗原诱导的收缩发生后给予FPL 55712会导致松弛。这些数据表明，组胺和SRS - A均参与致敏豚鼠和人气道组织对抗原的反应，其中组胺介导收缩的早期阶段，而SRS - A主要介导延长阶段。
