Li D C
Department of Pathology PUMC Hospital, CAMS, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1993 Apr;22(2):101-3.
By using 35S-dCTP labelled HPV16 probe, the HPV DNA sequence in 32 cases of condyloma acuminatum and suspicious condyloma acuminatum of vulva and vagina as well as 18 cases of papillomas at different anatomical sites were detected. The results showed that the HPV DNA sequences were positive in all 25 cases of condyloma acuminata which were typical both clinically and pathologically and in 6/7 of the suspicious cases; whereas in only 1/18 of the papillomas at various sites were they positive. Thus, nucleic acid hybridization-in-situ technique combined with histopathology seemed to be of great value for the diagnosis and differential diagnosis of condyloma acuminatum.
采用35S - dCTP标记的人乳头瘤病毒16型（HPV16）探针，检测32例外阴及阴道尖锐湿疣和可疑尖锐湿疣以及18例不同解剖部位乳头状瘤中的HPV DNA序列。结果显示，25例临床及病理均典型的尖锐湿疣病例以及7例可疑病例中的6例HPV DNA序列呈阳性；而不同部位的乳头状瘤中仅1/18呈阳性。因此，核酸原位杂交技术结合组织病理学检查对尖锐湿疣的诊断及鉴别诊断似乎具有重要价值。
