Nagai N, Kioka H, Ohta S, Fujimoto H, Tanimoto H, Fujii T, Fujiwara A
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Jul;42(7):655-61.
We investigated human papillomavirus (HPV) DNA of vulvar diseases obtained from 18 cases of condyloma acuminatum, 3 cases of hyperplastic dystrophy without atypia, 4 cases of lichen sclerosus, 4 cases of Bowenoid papulosis, one case of Bowen disease and 8 cases of squamous cell cancer by in situ hybridization with biotinylated HPV 6, 11, 16 and 18 DNA probes. The results of in situ hybridization analysis showed that HPV 6/11 was positive in 94.4% (17/18) of condyloma acuminatum. In 17 cases of HPV 6/11 positive condyloma acuminatum, 3 cases were positive for HPV 16 and one for HPV 18, respectively. HPV 16 was positive in 75.0% (3/4) of Bowenoid papulosis and one case of Bowen disease was positive for HPV 18. In two cases of Bowenoid papulosis which were positive for HPV 16, cervical carcinoma in situ containing HPV 16 DNA sequences was also found during the follow up period. In 8 cases of squamous cell cancer, 2 cases were positive for HPV 18, one for HPV 16 and one for HPV 6/11. All cases of hyperplastic dystrophy without atypia and lichen sclerosus were negative for HPV DNA. Our results suggested HPV is closely associated with vulvar neoplasia.
我们采用生物素化的人乳头瘤病毒（HPV）6、11、16和18型DNA探针，通过原位杂交技术，对18例尖锐湿疣、3例无非典型增生的增生性营养不良、4例硬化性苔藓、4例鲍温样丘疹病、1例鲍温病以及8例鳞状细胞癌的外阴疾病标本进行了HPV DNA检测。原位杂交分析结果显示，94.4%（17/18）的尖锐湿疣HPV 6/11呈阳性。在17例HPV 6/11阳性的尖锐湿疣中，分别有3例HPV 16阳性，1例HPV 18阳性。75.0%（3/4）的鲍温样丘疹病HPV 16呈阳性，1例鲍温病HPV 18呈阳性。在2例HPV 16阳性的鲍温样丘疹病患者随访期间，还发现了含HPV 16 DNA序列的宫颈原位癌。8例鳞状细胞癌中，2例HPV 18呈阳性，1例HPV 16呈阳性，1例HPV 6/11呈阳性。所有无非典型增生的增生性营养不良和硬化性苔藓病例HPV DNA均为阴性。我们的结果提示HPV与外阴肿瘤密切相关。
