Lindner V, Fingerle J, Reidy M A
Department of Pathology, University of Washington School of Medicine, Seattle.
Circ Res. 1993 Nov;73(5):792-6. doi: 10.1161/01.res.73.5.792.
In the present study, we established an injury model of the mouse carotid artery. Complete removal of the endothelium was achieved with a flexible wire. A platelet monolayer covered the denuded surface, and damage to underlying medial smooth muscle cells (SMCs) was detected. Injection of [3H]thymidine was used to determine the replication index for medial SMCs, which was found to be 1.6% at 2 days after denudation and 9.8% at 5 days. SMCs were observed in the intima by day 8 (replication index, 66%), and by 2 weeks the intimal lesion had a similar cell content as the media. In most animals, repair of the endothelial lining was complete 3 weeks after injury. The present model will allow us to use transgenic animals to address questions relevant to vascular biology and atherosclerosis.
在本研究中，我们建立了小鼠颈动脉损伤模型。用一根柔性金属丝实现了内皮的完全去除。血小板单层覆盖了剥脱表面，并检测到对其下方中膜平滑肌细胞（SMC）的损伤。注射[3H]胸腺嘧啶核苷用于确定中膜SMC的复制指数，发现剥脱后2天为1.6%，5天为9.8%。到第8天在内膜观察到SMC（复制指数为66%），到2周时内膜病变的细胞含量与中膜相似。在大多数动物中，损伤后3周内皮衬里的修复完成。本模型将使我们能够使用转基因动物来解决与血管生物学和动脉粥样硬化相关的问题。
