Gerloff C, Knappe U J, Hettmannsperger U, Duffner T K, Volk B
Neuropathologische Abteilung, Albert-Ludwigs-Universität Freiburg, Germany.
Brain Res Dev Brain Res. 1993 Jul 16;74(1):30-40. doi: 10.1016/0165-3806(93)90080-t.
Pieces of cerebellar primordia were obtained from G16 (day 16 of gestation) rat fetuses and stereotaxically injected into the striatum of adult Wistar rats. The transplants were allowed to integrate with the host brain for 2 h up to 6 months after implantation. Ninety four out of 105 transplants perfectly integrated with the host brain (90%) and established the typical trilaminar histoarchitecture of cerebellar cortex. The transplants were sufficiently vascularized. Vessels seen within the grafts provided all ultrastructural elements of a blood-brain barrier. Light microscopic evaluation of graft development showed no considerable retardation of cerebellar histogenesis. Electron microscopic examination disclosed normal ultrastructure of cerebellar neurons, as well as elements of regular synaptic organization. The topic of efferent graft-to-host projections was investigated 2.5 months after transplantation using the monoclonal Purkinje cell marker anti-Leu-4 (CD3). This method allowed us to detect immunoreactive, morphologically intact axons of grafted Purkinje cells running over long distances (at least 500 microns) within the host striatum. Whilst afferent but in no case efferent connections of heterotopic cerebellar transplants had been demonstrated elsewhere, we could now prove the reciprocal modus of graft-host interaction with heterotopic cerebellar grafts.
从小鼠胚胎第16天（G16）的胎儿获取小脑原基组织块，并通过立体定位注射到成年Wistar大鼠的纹状体中。移植后，让移植物与宿主脑整合2小时至6个月。105个移植物中有94个（90%）与宿主脑完美整合，并建立了典型的小脑皮质三层组织结构。移植物有充分的血管化。在移植物中观察到的血管具备血脑屏障的所有超微结构成分。对移植物发育的光学显微镜评估显示小脑组织发生没有明显延迟。电子显微镜检查揭示小脑神经元的超微结构正常，以及突触组织规则。在移植2.5个月后，使用抗Leu-4（CD3）单克隆浦肯野细胞标记物研究传出性移植物到宿主的投射。这种方法使我们能够检测到移植的浦肯野细胞的免疫反应性、形态完整的轴突在宿主纹状体内远距离（至少500微米）延伸。虽然异位小脑移植的传入连接而非传出连接在其他地方已有报道，但我们现在可以证明异位小脑移植物与宿主相互作用的双向模式。
