Bergmann M, Schuster T, Grabs D, Marquèze-Pouey B, Betz H, Traurig H, Mayerhofer A, Gratzl M
Institut für Anatomie, Charité, Humboldt-Universität zu Berlin, Germany.
Brain Res Dev Brain Res. 1993 Aug 20;74(2):235-44. doi: 10.1016/0165-3806(93)90009-y.
The expressions of two closely related synaptic vesicle antigens synaptophysin and synaptoporin were examined in the olfactory system of the adult rat and during pre- and postnatal development. In the adult, immunocytochemistry showed that the continuously regenerating olfactory receptor neurons (primary neurons) produce both synaptophysin and synaptoporin which were localized in the cell bodies of the receptor neurons in the olfactory epithelium, their dendrites, axonal processes in the olfactory nerve and their terminals in the olfactory bulb glomeruli. Furthermore, ultrastructural analysis revealed synaptophysin- and synaptoporin-immunoreactivities associated with synaptic vesicles in most olfactory receptor axonal terminals impinging on dendrites of the mitral and tufted neurons (secondary neurons in the olfactory bulb circuitry) in the olfactory glomeruli. In like manner, tufted neurons, granule and periglomerular neurons (interneurons in the olfactory bulb circuitry) express both synaptophysin and synaptoporin. In contrast, mitral neurons expressed only the synaptophysin antigen which was likewise associated with mitral axonal terminals in their target the olfactory cortex. The patterns of synaptophysin and synaptoporin expressions in mitral neurons (synaptophysin only) and tufted neurons (synaptophysin and synaptoporin) were similar in prenatal, postnatal and adult rats as revealed by immunocytochemistry and in situ hybridization. However, the biosynthesis of synaptophysin and synaptoporin by granule and periglomerular neurons, olfactory bulb interneurons, occurred mainly postnatally.
在成年大鼠的嗅觉系统以及产前和产后发育过程中，对两种密切相关的突触小泡抗原——突触素和突触孔蛋白的表达进行了研究。在成年大鼠中，免疫细胞化学显示，持续再生的嗅觉受体神经元（初级神经元）可产生突触素和突触孔蛋白，它们定位于嗅觉上皮中受体神经元的细胞体、其树突、嗅神经中的轴突以及嗅球小球中的终末。此外，超微结构分析显示，在大多数与嗅小球中二尖瓣神经元和簇状神经元（嗅球回路中的次级神经元）树突接触的嗅觉受体轴突终末中，突触素和突触孔蛋白免疫反应性与突触小泡相关。同样，簇状神经元、颗粒神经元和球周神经元（嗅球回路中的中间神经元）均表达突触素和突触孔蛋白。相比之下二尖瓣神经元仅表达突触素抗原，该抗原同样与它们在靶标嗅觉皮质中的轴突终末相关。免疫细胞化学和原位杂交显示，在产前、产后和成年大鼠中，二尖瓣神经元（仅突触素）和簇状神经元（突触素和突触孔蛋白）中突触素和突触孔蛋白的表达模式相似。然而，颗粒神经元和球周神经元（嗅球中间神经元）对突触素和突触孔蛋白的生物合成主要发生在出生后。
