Alvites Rui D, Branquinho Mariana V, Caseiro Ana R, Amorim Irina, Santos Pedrosa Sílvia, Rêma Alexandra, Faria Fátima, Porto Beatriz, Oliveira Cláudia, Teixeira Paula, Magalhães Rui, Geuna Stefano, Varejão Artur S P, Maurício Ana C
Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
Int J Cell Biol. 2020 Jan 29;2020:2938258. doi: 10.1155/2020/2938258. eCollection 2020.
Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.
基于干细胞/基质细胞的疗法是再生医学的一个分支，是促进受损或功能失调的组织和器官修复的一个有吸引力的选择。嗅黏膜间充质干细胞因其多能性、高增殖率、有利的位置以及较少的相关伦理问题等多种有利特性，被视为再生疗法中一种有前景的工具。这些细胞在包括大鼠在内的大多数哺乳动物的鼻腔中很容易获取，可轻松应用于自体治疗，并且不存在与使用其他干细胞相关的大多数障碍。尽管如此，由于迄今为止进行的研究数量较少，且不存在用于采集、分离和治疗应用的标准且明确的方案，其在临床试验以及人类和动物患者中的应用仍然有限。在本研究中，我们对大鼠模型的嗅黏膜间充质干细胞的分离、培养、扩增、冷冻和解冻方案进行了验证，并对这些细胞进行了生物学特性分析。为了研究嗅黏膜间充质干细胞的治疗潜力及其在临床试验中的最终安全应用，我们探讨了嗅黏膜间充质干细胞干性的主要特征。
