Ekong T, Gompels M, Clark C, Parkin J, Pinching A
Department of Immunology, St Mary's Hospital Medical School, Paddington, London, United Kingdom.
Cytometry. 1993;14(6):679-84. doi: 10.1002/cyto.990140613.
An unusual double-staining artefact has been repeatedly observed in certain individuals (both HIV-positive and healthy laboratory controls) during 2-colour immunophenotyping of lymphocytes by flow cytometry after red cell lysis. This artefact can falsely suggest co-expression of CD4 and CD8, as well as some of the other monoclonal antibody pairs commonly used in the typing of lymphocytes. It is caused by a serum factor associated with the ammonium-sulphate-precipitated and gamma-globulin fractions. It appears to be fairly common in the population (being found in 6 out of 105 of our healthy laboratory controls and in 42 out of 247 of our HIV-1 seropositive subjects); it may be genetically determined, but may be acquired in some. It is a potential source of error in lymphocyte immunophenotyping, which can be avoided by simple adjustments to the recommended techniques.
在红细胞裂解后通过流式细胞术对淋巴细胞进行双色免疫表型分析的过程中，在某些个体（包括HIV阳性者和健康的实验室对照）中反复观察到一种不寻常的双重染色假象。这种假象可能错误地提示CD4和CD8的共表达，以及淋巴细胞分型中常用的一些其他单克隆抗体对的共表达。它是由与硫酸铵沉淀的γ球蛋白组分相关的血清因子引起的。它在人群中似乎相当常见（在我们105名健康实验室对照中有6例发现，在247例HIV-1血清阳性受试者中有42例发现）；它可能由基因决定，但在某些情况下也可能后天获得。它是淋巴细胞免疫表型分析中一个潜在的误差来源，通过对推荐技术进行简单调整可以避免。
