维生素B12的生物合成。氧化环收缩和第四个甲基插入酶的发现。
Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group.
作者信息
Scott A I, Roessner C A, Stolowich N J, Spencer J B, Min C, Ozaki S I
机构信息
Department of Chemistry, Texas A&M University, College Station 77843-3255.
出版信息
FEBS Lett. 1993 Sep 27;331(1-2):105-8. doi: 10.1016/0014-5793(93)80306-f.
In the vitamin B12 biosynthetic pathway the enzymes responsible for the conversion of precorrin-3 to precorrin-4 have been identified as the gene products of cobG and cobJ from Pseudomonas denitrificans. CobG catalyzes the oxidation of precorrin-3 to precorrin-3x (a hydroxy lactone) whereas CobJ is a SAM-dependent C-17 methyl transferase and is necessary for ring contraction. A mechanism for ring contraction is proposed.
在维生素B12生物合成途径中，负责将前咕啉-3转化为前咕啉-4的酶已被鉴定为反硝化假单胞菌中cobG和cobJ的基因产物。CobG催化前咕啉-3氧化为前咕啉-3x（一种羟基内酯），而CobJ是一种依赖S-腺苷甲硫氨酸的C-17甲基转移酶，是环收缩所必需的。本文提出了一种环收缩机制。
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