Fujitani K, Sasaki-Tozawa N, Kikuchi Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
FEBS Lett. 1993 Sep 27;331(1-2):155-8. doi: 10.1016/0014-5793(93)80316-m.
The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.
发夹状核酶能高效切割RNA中5'GUC3'序列5'端的磷酸二酯键。具有5'GUA3'序列而非GUC序列的RNA是这种核酶的劣质底物。在此，我们表明在反式作用核酶系统中确实如此，但在顺式作用核酶系统中，这种核酶切割GUA序列5'端的效率与野生型切割GUC序列的效率相同。核酶中的一个碱基替换也影响了顺式作用系统中的靶位点特异性。
