Ansari K A, Rand A, Loch J A
J Neuropathol Exp Neurol. 1978 Nov-Dec;37(6):756-67. doi: 10.1097/00005072-197811000-00004.
Clinical and pathological studies have revealed that in multiple sclerosis (MS) the involvement of the optic tracts is much more frequent than that of the olfactory tracts. To investigate the possible reasons for this difference in involvement of these two adjacent structures, both containing a central type myelin, we have studied optic and olfactory tracts obtained at autopsy from 7 adult males ranging in age from 54 to 64 years. White matter from the frontal poles of the same individuals was used for reference. These tissues were compared with respect to the relative content of a) water, b) soluble proteins, c) 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) activity, and d) immunologically precipitable basic protein (BP). Homogenates from these tissues were further compared by disc gel electrophoresis in two systems; phenolformic acid-water and SDS-urea gels. Results indicate that while the optic tracts and the frontal pole white matter were similar with respect to their water, total protein content and BP content, the optic tracts had lower CNP activity than the frontal poles. The olfactory tracts contained more water and less BP and the CNP activity of these structures was lower than that of the frontal pole white matter. Assuming the CNP activity and the BP content are true measures of the total myelin content of a given tissue, it appears that olfactory tracts have smaller amounts of myelin. On the other hand, the optic tracts contain only half as much CNP-activity with a disproportionately greater amount of BP. The possible significance of these findings is discussed.
临床和病理研究表明，在多发性硬化症（MS）中，视束受累的频率远高于嗅束。为了探究这两个相邻结构（均含有中枢型髓磷脂）受累情况存在差异的可能原因，我们研究了7名年龄在54至64岁之间的成年男性尸检时获取的视束和嗅束。将同一患者额叶极的白质用作对照。对这些组织在以下方面进行了比较：a）水的相对含量、b）可溶性蛋白质、c）2'，3'-环核苷酸3'-磷酸水解酶（CNP）活性以及d）免疫可沉淀碱性蛋白（BP）。通过两种系统的圆盘凝胶电泳进一步比较了这些组织的匀浆；酚 - 甲酸 - 水凝胶和SDS - 尿素凝胶。结果表明，虽然视束和额叶极白质在水、总蛋白含量和BP含量方面相似，但视束的CNP活性低于额叶极。嗅束含有更多的水和更少的BP，并且这些结构的CNP活性低于额叶极白质。假设CNP活性和BP含量是给定组织总髓磷脂含量的真实指标，那么嗅束的髓磷脂含量似乎较少。另一方面，视束的CNP活性仅为一半，而BP含量却不成比例地更高。本文讨论了这些发现的可能意义。
