Swietnicki Wieslaw, Caspi Ron
Department of Immunology of Infectious Diseases, L. Hirszfeld Institute of Immunology and Experimental Therapy of PAS, ul. R. Weigla 12, 53-114 Wroclaw, Poland.
Artificial Intelligence Center, SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493, USA.
Pathogens. 2021 Mar 20;10(3):374. doi: 10.3390/pathogens10030374.
is an oral human pathogen. The bacterium destroys dental tissue and is a serious health problem worldwide. Experimental data and bioinformatic analysis revealed that the pathogen produces three types of lipopolysaccharides (LPS): normal (O-type), anionic (A-type), and capsular (K-type). The enzymes involved in the production of all three types of lipopolysaccharide have been largely identified for the first two and partially for the third type. In the current work, we use bioinformatics tools to predict biosynthetic pathways for the production of the normal (O-type) lipopolysaccharide in the W50 strain and compare the pathway with other putative pathways in fully sequenced and completed genomes of other pathogenic strains. Selected enzymes from the pathway have been modeled and putative structures are presented. The pathway for the A-type antigen could not be predicted at this time due to two mutually exclusive structures proposed in the literature. The pathway for K-type antigen biosynthesis could not be predicted either due to the lack of structural data for the antigen. However, pathways for the synthesis of lipid A, its core components, and the O-type antigen ligase reaction have been proposed based on a combination of experimental data and bioinformatic analyses. The predicted pathways are compared with known pathways in other systems and discussed. It is the first report in the literature showing, in detail, predicted pathways for the synthesis of selected LPS components for the model W50 strain of .
是一种口腔人类病原体。这种细菌会破坏牙齿组织，是全球范围内严重的健康问题。实验数据和生物信息学分析表明，该病原体产生三种类型的脂多糖（LPS）：正常型（O型）、阴离子型（A型）和荚膜型（K型）。前两种类型脂多糖生产所涉及的酶已基本确定，第三种类型部分确定。在当前工作中，我们使用生物信息学工具预测W50菌株中正常型（O型）脂多糖的生物合成途径，并将该途径与其他致病菌株全基因组测序完成后的其他假定途径进行比较。从该途径中选择的酶已进行建模并给出了假定结构。由于文献中提出了两种相互排斥的结构，目前无法预测A型抗原的途径。由于缺乏该抗原的结构数据，也无法预测K型抗原的生物合成途径。然而，基于实验数据和生物信息学分析的结合，已提出了脂质A及其核心成分的合成途径以及O型抗原连接酶反应。将预测的途径与其他系统中的已知途径进行比较并讨论。这是文献中首次详细展示针对模型W50菌株选定LPS成分合成的预测途径的报告。
