Pilder S H, Olds-Clarke P, Phillips D M, Silver L M
Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Dev Biol. 1993 Oct;159(2):631-42. doi: 10.1006/dbio.1993.1270.
Mouse t haplotypes alter sperm differentiation, resulting in abnormal sperm movement and sterility. In previous studies, a locus responsible for hybrid sterility in the genus Mus,Hst-4, was mapped to the distal inversion of the t complex on chromosome 17. Here we report the identification and characterization of two additional hybrid sterility loci, Hst-5 and Hst-6, that map to the same inversion. We further show that an abnormality in sperm flagellar curvature deriving from interactions between t haplotypes and the M. spretus allele of Hst-6 is indistinguishable from one exhibited by sperm from mice carrying two t haplotypes. Additionally, we demonstrate that this latter phenotype maps to the distal inversion of t haplotypes. Morphological and functional studies of Hst-6 mutant sperm also imply that the product(s) of Hst-6 is a spermatogenic-specific protein, important for assembly and function of the sperm axoneme. Thus, Hst-6 provides direct access to the molecular basis of t haplotype-specific alterations in sperm function that emanate from the t complex distal inversion.
小鼠t单倍型会改变精子分化，导致精子运动异常和不育。在之前的研究中，一个负责小家鼠属杂交不育的基因座Hst - 4，被定位到17号染色体上t复合体的远端倒位区域。在此，我们报告另外两个杂交不育基因座Hst - 5和Hst - 6的鉴定与特征，它们也定位在同一倒位区域。我们进一步表明，由于t单倍型与Hst - 6的西班牙小鼠等位基因之间的相互作用而导致的精子鞭毛弯曲异常，与携带两个t单倍型的小鼠精子所表现出的异常无法区分。此外，我们证明后一种表型定位到t单倍型的远端倒位区域。对Hst - 6突变精子的形态学和功能研究还表明，Hst - 6的产物是一种精子发生特异性蛋白，对精子轴丝的组装和功能很重要。因此，Hst - 6为深入了解源自t复合体远端倒位的t单倍型特异性精子功能改变的分子基础提供了直接途径。
