Harrison A, Olds-Clarke P, King S M
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032-3305, USA.
J Cell Biol. 1998 Mar 9;140(5):1137-47. doi: 10.1083/jcb.140.5.1137.
The cytoplasmic dynein light chain Tctex1 is a candidate for one of the distorter products involved in the non-Mendelian transmission of mouse t haplotypes. It has been unclear, however, how the t-specific mutations in this protein, which is found associated with cytoplasmic dynein in many tissues, could result in a male germ cell-specific phenotype. Here, we demonstrate that Tctex1 is not only a cytoplasmic dynein component, but is also present both in mouse sperm and Chlamydomonas flagella. Genetic and biochemical dissection of the Chlamydomonas flagellum reveal that Tctex1 is a previously undescribed component of inner dynein arm I1. Combined with the recent identification of another putative t complex distorter, Tctex2, within the outer dynein arm, these results support the hypothesis that transmission ratio distortion (meiotic drive) of mouse t haplotypes involves dysfunction of both flagellar inner and outer dynein arms but does not require the cytoplasmic isozyme.
细胞质动力蛋白轻链Tctex1是参与小鼠t单倍型非孟德尔遗传的畸变产物之一的候选者。然而，尚不清楚在许多组织中发现与细胞质动力蛋白相关的这种蛋白质中的t特异性突变如何导致雄性生殖细胞特异性表型。在这里，我们证明Tctex1不仅是细胞质动力蛋白的一个组成部分，而且也存在于小鼠精子和衣藻鞭毛中。对衣藻鞭毛的遗传和生化分析表明，Tctex1是内动力蛋白臂I1中一个以前未描述的组成部分。结合最近在外动力蛋白臂中鉴定出另一种假定的t复合畸变蛋白Tctex2，这些结果支持了这样一种假说，即小鼠t单倍型的传递比率畸变（减数分裂驱动）涉及鞭毛内、外动力蛋白臂的功能障碍，但不需要细胞质同工酶。
