Gao B, Kunos G
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Gene. 1993 Sep 15;131(2):243-7. doi: 10.1016/0378-1119(93)90300-r.
Using a rat alpha 1B adrenergic receptor(AR)-encoding cDNA probe, we isolated two genomic clones from a rat liver genomic DNA library. Southern blot analysis and nucleotide sequencing indicate that the rat alpha 1B AR gene has two exons and a single large intron of at least 16 kb. Analysis of the sequence of the 5'-flanking region suggests that this gene has the features of a housekeeping gene: it has neither a TATA box nor a CAAT box, but has multiple transcription start points (tsp), multiple Sp1-binding sites, and a high G + C content. The 5'-flanking region also contains consensus sequences for AP1- and AP2-binding sites, and putative sites for cyclic AMP (CRE), glucocorticoid (GRE), and thyroid responses (TRE). The 3'-flanking region contains a putative polyadenylation signal (ATTAAA) 492 bp downstream from the stop codon. A comparison of the rat alpha 1B AR sequence with that of the recently cloned human alpha 1B AR gene showed that the overall structure of this gene is highly conserved, with some notable differences in the 5'-flanking region.
利用大鼠α1B肾上腺素能受体（AR）编码的cDNA探针，我们从大鼠肝脏基因组DNA文库中分离出两个基因组克隆。Southern印迹分析和核苷酸测序表明，大鼠α1B AR基因有两个外显子和一个至少16 kb的单一大型内含子。对5'-侧翼区序列的分析表明，该基因具有管家基因的特征：它既没有TATA盒也没有CAAT盒，但有多个转录起始点（tsp）、多个Sp1结合位点，以及高G + C含量。5'-侧翼区还包含AP1和AP2结合位点的共有序列，以及环磷酸腺苷（CRE）、糖皮质激素（GRE）和甲状腺反应（TRE）的假定位点。3'-侧翼区在终止密码子下游492 bp处包含一个假定的多聚腺苷酸化信号（ATTAAA）。将大鼠α1B AR序列与最近克隆的人类α1B AR基因序列进行比较，结果表明该基因的整体结构高度保守，不过在5'-侧翼区存在一些显著差异。
