Kondo S, Isobe K, Ishiguro N, Nakashima I, Miura T
Laboratory of Orthopaedic Surgery, Nagoya University School of Medicine, Aichi, Japan.
Immunology. 1993 Jul;79(3):459-64.
We investigated the effects of transforming growth factor-beta 1 (TGF-beta 1) on the proliferation and generation of murine T lymphocytes in vitro. TGF-beta 1 suppressed T- and B-lymphocyte proliferation, mixed lymphocyte reaction (MLR), and the generation of natural killer (NK) cells and lymphokine-activated killer (LAK) cells. On the other hand, TGF-beta 1 significantly enhanced the generation of allospecific cytotoxic T lymphocytes (CTL) at low concentrations (0.01-1 ng/ml) in a dose-dependent manner and restored it to the control level at higher concentrations (10-40 ng/ml). Allospecific CTL generation by TGF-beta 1 was maximally enhanced when added at the beginning of culture. Less enhancement occurred when the addition was delayed. Anti-TGF-beta 1 antibody completely abolished the enhancing effects of TGF-beta 1. Furthermore, platelet-derived TGF-beta (pTGF-beta) as well as recombinant TGF-beta 1 similarly enhanced the generation of allospecific CTL. These data demonstrate that TGF-beta has not only immunosuppressive effects but also immuno-enhancing effects in vitro.
我们研究了转化生长因子-β1（TGF-β1）对小鼠T淋巴细胞体外增殖和生成的影响。TGF-β1抑制T淋巴细胞和B淋巴细胞增殖、混合淋巴细胞反应（MLR）以及自然杀伤（NK）细胞和淋巴因子激活的杀伤（LAK）细胞的生成。另一方面，TGF-β1在低浓度（0.01 - 1 ng/ml）时以剂量依赖方式显著增强同种异体特异性细胞毒性T淋巴细胞（CTL）的生成，并在高浓度（10 - 40 ng/ml）时将其恢复到对照水平。当在培养开始时添加TGF-β1时，同种异体特异性CTL生成的增强作用最大。添加延迟时增强作用较小。抗TGF-β1抗体完全消除了TGF-β1的增强作用。此外，血小板衍生的TGF-β（pTGF-β）以及重组TGF-β1同样增强了同种异体特异性CTL的生成。这些数据表明，TGF-β在体外不仅具有免疫抑制作用，还具有免疫增强作用。
