Shalaby M R, Ammann A J
Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080.
Cell Immunol. 1988 Apr 1;112(2):343-50. doi: 10.1016/0008-8749(88)90303-6.
The influence of recombinant human transforming growth factor-beta (rHuTGF-beta) on B-cell function and antigen-specific T-cell responses in vitro was investigated. The addition of 0.1 ng/ml of rHuTGF-beta to cultures of peripheral blood mononuclear cells (PBMC) stimulated with tetanus toxoid resulted in a 50% inhibition of the PBMC proliferative response as determined by [3H]thymidine incorporation. Further, rHuTGF-beta at 0.37 ng/ml caused a greater than 50% reduction in the number of immunoglobulin G-secreting cells among PBMC induced by pokeweed mitogen. rHuTGF-beta also inhibited the generation of allospecific cytotoxic T lymphocytes (CTL) in the mixed-lymphocyte reaction but had no effect on the cytolytic function of CTL generated in the absence of exogenous HuTGF-beta. The results indicate additional immunoregulatory activities for HuTGF-beta and suggest that this factor may play an important role in the regulation of the antigen-dependent immune response.
研究了重组人转化生长因子-β（rHuTGF-β）对体外B细胞功能和抗原特异性T细胞反应的影响。将0.1 ng/ml的rHuTGF-β添加到用破伤风类毒素刺激的外周血单个核细胞（PBMC）培养物中，通过[3H]胸苷掺入法测定，PBMC增殖反应受到50%的抑制。此外，0.37 ng/ml的rHuTGF-β使商陆有丝分裂原诱导的PBMC中分泌免疫球蛋白G的细胞数量减少超过50%。rHuTGF-β还抑制了混合淋巴细胞反应中同种异体特异性细胞毒性T淋巴细胞（CTL）的产生，但对外源HuTGF-β不存在时产生的CTL的细胞溶解功能没有影响。结果表明HuTGF-β具有额外的免疫调节活性，并提示该因子可能在抗原依赖性免疫反应的调节中起重要作用。
