微管抑制剂可阻断微小隐孢子虫对人肠上皮细胞系的感染。
Microtubule inhibitors block Cryptosporidium parvum infection of a human enterocyte cell line.
作者信息
Wiest P M, Johnson J H, Flanigan T P
机构信息
International Health Institute, Miriam Hospital, Brown University, Providence, Rhode Island 02906.
出版信息
Infect Immun. 1993 Nov;61(11):4888-90. doi: 10.1128/iai.61.11.4888-4890.1993.
No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting drugs. The number of parasites per 1,000 cells was reduced by 77% (P < 0.001, n = 4) from 182 +/- 3 in untreated cells to 42 +/- 4 in cells treated with 10(-4) M colchicine. Inhibition of C. parvum infection was concentration dependent. Similar results were seen with a second microtubular depolymerization agent, vinblastine. These data suggest that microtubules are important in host cell invasion by C. parvum and may represent targets for development of new therapeutic drugs for treatment of cryptosporidiosis.
微小隐孢子虫是一种球虫类原生动物寄生虫，可导致艾滋病患者严重腹泻，目前尚无有效的治疗方法。通过在微管破坏药物存在的情况下，将10⁷个卵囊与HT 29.74细胞系孵育24小时，研究了微管在寄生虫侵入宿主细胞中的作用。每1000个细胞中的寄生虫数量从未处理细胞中的182±3减少了77%（P<0.001，n = 4），在用10⁻⁴M秋水仙碱处理的细胞中降至42±4。微小隐孢子虫感染的抑制呈浓度依赖性。用第二种微管解聚剂长春碱也观察到了类似结果。这些数据表明，微管在微小隐孢子虫侵入宿主细胞中起重要作用，可能是开发治疗隐孢子虫病新治疗药物的靶点。
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