Li S L, Feichtinger H, Kaaya E, Migliorini P, Putkonen P, Biberfeld G, Middeldorp J M, Biberfeld P, Ernberg I
Immunopathology Laboratory, Karolinska Institute, Stockholm, Sweden.
Int J Cancer. 1993 Oct 21;55(4):609-15. doi: 10.1002/ijc.2910550416.
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
感染猿猴免疫缺陷病毒（SIV）的食蟹猴约三分之一会发生B细胞淋巴瘤。我们现已研究了13例携带食蟹猴爱泼斯坦-巴尔病毒（cyno-EBV）的与SIVsm相关的猴淋巴瘤中食蟹猴EBV核抗原的表达情况，并从其中3个肿瘤建立了细胞系。用针对人EB病毒EBNAI-6以及膜蛋白LMP1的多特异性和单特异性试剂探测细胞裂解物的免疫印迹。在8个淋巴瘤组织（8/13）以及源自肿瘤的3个细胞系中证实了一种EBNA2交叉反应蛋白。所有肿瘤均表达一种分子量为50至55 kDa的多肽，该多肽与一些抗EBNAI抗体发生交叉反应。用正常猴组织进行的吸收实验表明，这种多肽对携带cyno-EBV的淋巴瘤细胞具有特异性。未检测到相当于EBNA3-6和LMP1的物质。免疫表型特征显示，猴淋巴瘤与人类HIV相关B细胞淋巴瘤相似。实验性感染SIVsm的食蟹猴中的恶性B细胞淋巴瘤可作为免疫缺陷状态下EBV相关淋巴瘤发生的模型。
