脂质修饰前脂蛋白的加工在体内需要能量和sec基因产物。
Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo.
作者信息
Kosic N, Sugai M, Fan C K, Wu H C
机构信息
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
出版信息
J Bacteriol. 1993 Oct;175(19):6113-7. doi: 10.1128/jb.175.19.6113-6117.1993.
Abstract
The kinetics of processing of glyceride-modified prolipoprotein that accumulated in globomycin-treated Escherichia coli has been found to be affected by sec mutations, i.e., secA, secE, secY, secD, and secF, and by metabolic poisons which affect proton motive force (PMF). The effect of sec mutations on processing of glyceride-modified prolipoprotein in vivo was not due to a secondary effect on PMF. Neither a secF mutation nor metabolic poisons affected the processing of previously accumulated proOmpA protein in vivo, suggesting that the requirements for functional sec gene products and PMF are specific to the processing of lipoprotein precursors by signal peptidase II.
摘要
已发现，在球状霉素处理的大肠杆菌中积累的甘油酯修饰前脂蛋白的加工动力学受sec突变（即secA、secE、secY、secD和secF）以及影响质子动力（PMF）的代谢毒物影响。sec突变对体内甘油酯修饰前脂蛋白加工的影响并非源于对PMF的次级效应。secF突变和代谢毒物均未影响体内先前积累的前OmpA蛋白的加工，这表明功能性sec基因产物和PMF的需求是信号肽酶II加工脂蛋白前体所特有的。
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