酿酒酵母的Rad1和Rad10蛋白作为一个复合体参与紫外线辐射损伤的核苷酸切除修复的证据。
Evidence that the Rad1 and Rad10 proteins of Saccharomyces cerevisiae participate as a complex in nucleotide excision repair of UV radiation damage.
作者信息
Siede W, Friedberg A S, Friedberg E C
机构信息
Department of Pathology, University of Texas, Southwestern Medical Center at Dallas 75235.
出版信息
J Bacteriol. 1993 Oct;175(19):6345-7. doi: 10.1128/jb.175.19.6345-6347.1993.
Abstract
A newly characterized rad1 missense mutation (rad1-20) in the yeast Saccharomyces cerevisiae maps to a region of the Rad1 polypeptide known to be required for Rad1-Rad10 complex formation. The UV sensitivity of the rad1-20 mutant can be partially and specifically corrected by overexpression of wild-type Rad10 protein. These results suggest that complex formation between the Rad1 and Rad10 proteins is required for nucleotide excision repair.
摘要
酿酒酵母中一个新鉴定的rad1错义突变（rad1-20）定位于已知Rad1-Rad10复合物形成所需的Rad1多肽区域。rad1-20突变体对紫外线的敏感性可通过野生型Rad10蛋白的过表达得到部分且特异性的纠正。这些结果表明，Rad1和Rad10蛋白之间的复合物形成是核苷酸切除修复所必需的。
相似文献
1
Evidence that the Rad1 and Rad10 proteins of Saccharomyces cerevisiae participate as a complex in nucleotide excision repair of UV radiation damage.酿酒酵母的Rad1和Rad10蛋白作为一个复合体参与紫外线辐射损伤的核苷酸切除修复的证据。
J Bacteriol. 1993 Oct;175(19):6345-7. doi: 10.1128/jb.175.19.6345-6347.1993.
2
Nucleotide-excision repair of DNA in cell-free extracts of the yeast Saccharomyces cerevisiae.酿酒酵母无细胞提取物中DNA的核苷酸切除修复
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4907-11. doi: 10.1073/pnas.90.11.4907.
3
Yeast DNA recombination and repair proteins Rad1 and Rad10 constitute a complex in vivo mediated by localized hydrophobic domains.酵母DNA重组与修复蛋白Rad1和Rad10在体内由局部疏水结构域介导形成一个复合体。
Mol Microbiol. 1993 Jun;8(6):1177-88. doi: 10.1111/j.1365-2958.1993.tb01662.x.
4
Yeast Tdp1 and Rad1-Rad10 function as redundant pathways for repairing Top1 replicative damage.酵母Tdp1和Rad1-Rad10作为修复Top1复制损伤的冗余途径发挥作用。
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13669-74. doi: 10.1073/pnas.202242599. Epub 2002 Oct 4.
5
Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo.与损伤识别蛋白Rad14形成复合物对于酿酒酵母Rad1-Rad10核酸酶在体内核苷酸切除修复中发挥其功能至关重要。
Mol Cell Biol. 2006 Feb;26(3):1135-41. doi: 10.1128/MCB.26.3.1135-1141.2006.
6
Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10.酵母DNA修复与重组基因RAD1和RAD10编码的蛋白质之间形成特定复合物。
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8273-7. doi: 10.1073/pnas.89.17.8273.
7
Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease.酵母Rad1-Rad10 DNA核酸内切酶对模型重组和修复中间体的特异性切割。
Science. 1994 Sep 30;265(5181):2082-5. doi: 10.1126/science.8091230.
8
Purification of Rad1 protein from Saccharomyces cerevisiae and further characterization of the Rad1/Rad10 endonuclease complex.从酿酒酵母中纯化Rad1蛋白并进一步鉴定Rad1/Rad10核酸内切酶复合物。
Biochemistry. 1994 May 3;33(17):5305-11. doi: 10.1021/bi00183a038.
9
Purification and characterization of the Saccharomyces cerevisiae RAD1/RAD10 endonuclease.酿酒酵母RAD1/RAD10核酸内切酶的纯化与特性分析
J Biol Chem. 1993 Dec 15;268(35):26391-9.
10
Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.酵母重组与DNA修复蛋白RAD1和RAD10在体外的稳定且特异性结合
Mol Cell Biol. 1992 Jul;12(7):3041-9. doi: 10.1128/mcb.12.7.3041-3049.1992.
引用本文的文献
1
Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae.Rad1、rad10和rad52突变会减少酿酒酵母在辐射诱导的微同源性介导的非法重组过程中微同源性长度的增加。
Radiat Res. 2009 Aug;172(2):141-51. doi: 10.1667/RR1675.1.
2
End resection initiates genomic instability in the absence of telomerase.在缺乏端粒酶的情况下，末端切除会引发基因组不稳定。
Mol Cell Biol. 2003 Dec;23(23):8450-61. doi: 10.1128/MCB.23.23.8450-8461.2003.
3
Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of srs2Delta with other DNA repair genes in Saccharomyces cerevisiae.重组修复和检查点控制基因中的突变抑制了酿酒酵母中srs2Delta与其他DNA修复基因的致死组合。
Genetics. 2001 Feb;157(2):557-65. doi: 10.1093/genetics/157.2.557.
4
The Arabidopsis UVH1 gene is a homolog of the yeast repair endonuclease RAD1.拟南芥UVH1基因是酵母修复内切核酸酶RAD1的同源物。
Plant Physiol. 2000 Oct;124(2):579-86. doi: 10.1104/pp.124.2.579.
5
Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes.酵母中有丝分裂同源重组的调控。错配修复和核苷酸切除修复基因的功能。
Genetics. 2000 Jan;154(1):133-46. doi: 10.1093/genetics/154.1.133.
6
Mutational analysis of the human nucleotide excision repair gene ERCC1.人类核苷酸切除修复基因ERCC1的突变分析
Nucleic Acids Res. 1996 Sep 1;24(17):3370-80. doi: 10.1093/nar/24.17.3370.
7
Nucleotide excision repair in yeast.酵母中的核苷酸切除修复
Curr Genet. 1994 Dec;27(1):1-16. doi: 10.1007/BF00326572.
8
Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosity.通过RAD51过表达、srs2和ccr4中的突变或交配型杂合性抑制酵母RAD52基因的一个新等位基因。
Genetics. 1995 May;140(1):115-27. doi: 10.1093/genetics/140.1.115.
本文引用的文献
1
Nucleotide-excision repair of DNA in cell-free extracts of the yeast Saccharomyces cerevisiae.酿酒酵母无细胞提取物中DNA的核苷酸切除修复
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4907-11. doi: 10.1073/pnas.90.11.4907.
2
Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease.酵母DNA修复和重组蛋白Rad1和Rad10构成一种单链DNA内切核酸酶。
Nature. 1993 Apr 29;362(6423):860-2. doi: 10.1038/362860a0.
3
Yeast DNA recombination and repair proteins Rad1 and Rad10 constitute a complex in vivo mediated by localized hydrophobic domains.酵母DNA重组与修复蛋白Rad1和Rad10在体内由局部疏水结构域介导形成一个复合体。
Mol Microbiol. 1993 Jun;8(6):1177-88. doi: 10.1111/j.1365-2958.1993.tb01662.x.
4
Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae.酿酒酵母切除缺陷型突变体中嘧啶二聚体去除的切割及切割后步骤。
J Bacteriol. 1981 Nov;148(2):618-23. doi: 10.1128/jb.148.2.618-623.1981.
5
Molecular cloning of eucaryotic genes required for excision repair of UV-irradiated DNA: isolation and partial characterization of the RAD3 gene of Saccharomyces cerevisiae.紫外线照射DNA切除修复所需真核基因的分子克隆：酿酒酵母RAD3基因的分离与部分特性分析
J Bacteriol. 1982 Oct;152(1):323-31. doi: 10.1128/jb.152.1.323-331.1982.
6
Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene.
Mol Cell Biol. 1984 Oct;4(10):2161-9. doi: 10.1128/mcb.4.10.2161-2169.1984.
7
Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.酵母RAD10基因的分子克隆与特性分析以及RAD10蛋白在大肠杆菌中的表达
EMBO J. 1985 Jun;4(6):1575-82. doi: 10.1002/j.1460-2075.1985.tb03819.x.
8
Overexpression of the RAD2 gene of S. cerevisiae: identification and preliminary characterization of Rad2 protein.酿酒酵母RAD2基因的过表达：Rad2蛋白的鉴定与初步表征
Yeast. 1987 Sep;3(3):149-60. doi: 10.1002/yea.320030303.
9
RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.RAD1是酿酒酵母的一种切除修复基因，它也参与重组过程。
Mol Cell Biol. 1988 Sep;8(9):3619-26. doi: 10.1128/mcb.8.9.3619-3626.1988.
10
Different types of recombination events are controlled by the RAD1 and RAD52 genes of Saccharomyces cerevisiae.酿酒酵母的RAD1和RAD52基因控制着不同类型的重组事件。
Genetics. 1988 Oct;120(2):367-77. doi: 10.1093/genetics/120.2.367.
Zotero 插件