Kauert G, Herrle I, Wermeille M
Institute of Legal Medicine, University of Munich, Germany.
J Chromatogr. 1993 Aug 11;617(2):318-23. doi: 10.1016/0378-4347(93)80506-y.
A gas chromatographic-mass fragmentographic method using ammonia chemical ionization for the determination of dimethindene in human plasma is described. The drug was isolated from plasma by liquid-liquid extraction with hexane-2-methylbutanol. Plasma components were separated on a capillary column coated with chemically bonded methyl silicone. For detection of dimethindene, its quasi-molecular ion (M + H+) was mass fragmentographically monitored after chemical ionization with ammonia as reagent gas. Dimethindene was quantified using methaqualone as the internal standard: the quantification limit in plasma was 0.2 ng/ml, the within-run precision was 8.0% and the inter-run precision 5.6%. The plasma concentration-time profile was established after a single dose of 4 mg of dimethindene with an average maximum concentration of 5.5 ng/ml, detectable up to 48 h post application.
本文描述了一种采用氨化学电离的气相色谱-质谱碎片分析法，用于测定人血浆中的二甲茚定。该药物通过用己烷-2-甲基丁醇进行液-液萃取从血浆中分离出来。血浆成分在涂有化学键合甲基硅氧烷的毛细管柱上进行分离。为了检测二甲茚定，以氨作为反应气进行化学电离后，对其准分子离子（M + H+）进行质谱碎片监测。使用甲喹酮作为内标对二甲茚定进行定量：血浆中的定量限为0.2 ng/ml，批内精密度为8.0%，批间精密度为5.6%。在单次服用4 mg二甲茚定后建立了血浆浓度-时间曲线，平均最大浓度为5.5 ng/ml，给药后48小时内均可检测到。
