Yoshimura T, Manabe T, Suwa H, Imamura T, Wang Z, Ohshio G, Yamabe H, Matsumoto M, Ogasahara K, Takasan H
First Department of Surgery, Kyoto University, Japan.
Int J Pancreatol. 1993 Aug;14(1):29-36. doi: 10.1007/BF02795227.
Eighty-six patients with carcinoma of the pancreas were studied retrospectively. Paraffin-embedded specimens and flow cytometry were used to evaluate the accuracy of the measurement of nuclear DNA as a predictor of the postoperative prognosis. From the series of 86 patients, 72 with a diagnosis of tubular adenocarcinoma (Japanese classification) were selected, and their DNA ploidy pattern and clinical and pathological features were correlated; 52.3% of the 86 patients and 52.8% of the 72 tubular adenocarcinoma patients showed DNA aneuploidy. Histological examinations of the tubular adenocarcinomas showed 42.9% DNA aneuploidy in well differentiated, 56.8% in moderately differentiated, and 71.4% in poorly differentiated types. The DNA ploidy showed a statistically significant positive correlation with the T category. The presence or absence of retroperitoneal invasion was thought to be the most important prognostic factor. Cumulative survival rates showed that the prognosis for patients with retroperitoneal invasion and DNA aneuploidy was significantly worse than for those with DNA diploidy or those without retroperitoneal invasion.
对86例胰腺癌患者进行了回顾性研究。采用石蜡包埋标本和流式细胞术评估核DNA测量作为术后预后预测指标的准确性。从这86例患者中，选取了72例诊断为管状腺癌（日本分类）的患者，将其DNA倍体模式与临床和病理特征进行关联分析；86例患者中的52.3%以及72例管状腺癌患者中的52.8%表现为DNA非整倍体。对管状腺癌的组织学检查显示，高分化型中DNA非整倍体占42.9%，中分化型占56.8%，低分化型占71.4%。DNA倍体与T分期呈统计学显著正相关。是否存在腹膜后侵犯被认为是最重要的预后因素。累积生存率显示，有腹膜后侵犯且DNA非整倍体的患者预后明显差于DNA二倍体患者或无腹膜后侵犯的患者。
