Ogura H, Watanabe S, Fukushima K, Masuda Y, Fujiwara T, Yabe Y
Department of Molecular Virology, Okayama University Medical School.
Jpn J Clin Oncol. 1993 Aug;23(4):221-5.
The prevalence of human papillomavirus (HPV) types 16 and 18 in clinical samples of squamous cell carcinomas from respiratory and upper digestive tracts was studied. HPV DNA of types 16 and/or 18 was detected using the polymerase chain reaction (PCR) method in 16 out of 121 cases (13.2%). By Southern blot hybridization, however, only the DNA from a laryngeal and a tonsillar carcinoma was found to hybridize with the whole HPV 16 DNA probe (two out of 16 HPV DNA-positive cases by PCR, 12.5%). None of the DNAs hybridized with the whole HPV 18 DNA probe. The discrepancy in the results of PCR and Southern blot hybridization methods seemed to reflect their sensitivity. The possible relation between prevalence of HPV DNA and carcinogenesis in respiratory and upper digestive tract is discussed.
对呼吸道和上消化道鳞状细胞癌临床样本中16型和18型人乳头瘤病毒（HPV）的流行情况进行了研究。采用聚合酶链反应（PCR）方法在121例病例中的16例（13.2%）检测到16型和/或18型HPV DNA。然而，通过Southern印迹杂交，仅发现来自1例喉癌和1例扁桃体癌的DNA与完整的HPV 16 DNA探针杂交（在PCR检测为HPV DNA阳性的16例病例中有2例，12.5%）。没有DNA与完整的HPV 18 DNA探针杂交。PCR和Southern印迹杂交方法结果的差异似乎反映了它们的敏感性。讨论了HPV DNA流行率与呼吸道和上消化道致癌作用之间的可能关系。
