Séité P, Hillion J, d'Agay M F, Berger R, Larsen C J
Unité 301 INSERM, Institut de Génétique Moléculaire, Paris, France.
Oncogene. 1993 Nov;8(11):3073-80.
Two rearrangements affecting the same allele of the BCL2 gene were characterized by molecular analysis of an untreated follicular lymphoma. The first rearrangement interested the major breakpoint region (mbr) on chromosome 18 and a JH segment on chromosome 14. The other one was located at the 5' end of the BCL2 gene, in the so called variant cluster region (vcr), and consisted of a series of deletions that removed part of a DNA region where initiation of transcription normally occurs. Interestingly, both rearrangements involved the same BCL2 allele. The simultaneous presence of mbr (or mcr) translocations and of minor rearrangements in vcr has been previously suggested by restriction map analysis in a significant number of follicular lymphomas. The significance of these abnormalities on the oncogenic process is discussed.
通过对未经治疗的滤泡性淋巴瘤进行分子分析，鉴定出影响BCL2基因同一等位基因的两种重排。第一种重排涉及18号染色体上的主要断裂点区域（mbr）和14号染色体上的一个JH片段。另一种位于BCL2基因的5'端，即所谓的可变簇区域（vcr），由一系列缺失组成，这些缺失去除了通常发生转录起始的部分DNA区域。有趣的是，两种重排都涉及同一个BCL2等位基因。先前通过对大量滤泡性淋巴瘤的限制性图谱分析表明，vcr中同时存在mbr（或mcr）易位和微小重排。讨论了这些异常在致癌过程中的意义。
