布氏锥虫微小环编码多个向导RNA，这些向导RNA可指导对广泛重叠序列的编辑。
Trypanosoma brucei minicircles encode multiple guide RNAs which can direct editing of extensively overlapping sequences.
作者信息
Corell R A, Feagin J E, Riley G R, Strickland T, Guderian J A, Myler P J, Stuart K
机构信息
Seattle Biomedical Research Institute, WA 98109-1651.
出版信息
Nucleic Acids Res. 1993 Sep 11;21(18):4313-20. doi: 10.1093/nar/21.18.4313.
Abstract
Small guide RNAs (gRNAs) may direct RNA editing in kinetoplastid mitochondria. We have characterized multiple gRNA genes from Trypanosoma brucei (EATRO 164), that can specify up to 30% of the editing of the COIII, ND7, ND8, and A6 mRNAs and we have also found that the non-translated region of edited COIII mRNA of strain (EATRO 164) differs from that of another strain. Several of the gRNAs specify overlapping regions of the same mRNA often specifying sequence beyond that required for an anchor duplex with the next gRNA. Some gRNAs have different sequence but specify identical editing of the same region of mRNA. These data indicate a complex gRNA population and consequent complex pattern of editing in T. brucei.
摘要
小向导RNA（gRNAs）可能指导动基体线粒体中的RNA编辑。我们已经对来自布氏锥虫（EATRO 164）的多个gRNA基因进行了表征，这些基因可以指定COIII、ND7、ND8和A6 mRNA高达30%的编辑，并且我们还发现菌株（EATRO 164）编辑后的COIII mRNA的非翻译区与另一菌株的不同。一些gRNAs指定同一mRNA的重叠区域，通常指定的序列超出了与下一个gRNA形成锚定双链体所需的序列。一些gRNAs具有不同的序列，但指定mRNA同一区域的相同编辑。这些数据表明布氏锥虫中存在复杂的gRNA群体以及随之而来的复杂编辑模式。
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