High efficiency transformation of Saccharomyces cerevisiae by electroporation.
作者信息
Manivasakam P, Schiestl R H
机构信息
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA 02115.
出版信息
Nucleic Acids Res. 1993 Sep 11;21(18):4414-5. doi: 10.1093/nar/21.18.4414.
Abstract
摘要
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本文引用的文献
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New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.用缺乏六碱基对限制位点的体外诱变酵母基因构建的新型酵母-大肠杆菌穿梭载体。
Gene. 1988 Dec 30;74(2):527-34. doi: 10.1016/0378-1119(88)90185-0.
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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.使用单链核酸作为载体对完整酵母细胞进行高效转化。
Curr Genet. 1989 Dec;16(5-6):339-46. doi: 10.1007/BF00340712.
3
High-efficiency transformation of yeast by electroporation.通过电穿孔实现酵母的高效转化。
Methods Enzymol. 1991;194:182-7. doi: 10.1016/0076-6879(91)94015-5.
4
The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.双杂交系统：一种用于鉴定和克隆与感兴趣蛋白质相互作用的蛋白质基因的方法。
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9578-82. doi: 10.1073/pnas.88.21.9578.
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Improved method for high efficiency transformation of intact yeast cells.完整酵母细胞高效转化的改进方法。
Nucleic Acids Res. 1992 Mar 25;20(6):1425. doi: 10.1093/nar/20.6.1425.
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