Bécuwe P, Slomianny C, Camus D, Dive D
INSERM U42, Domaine du CERTIA, Villeneuve d'Ascq, France.
Parasitol Res. 1993;79(5):349-52. doi: 10.1007/BF00931821.
Superoxide dismutase (SOD) was investigated in three species of rodent malaria (Plasmodium berghei, P. yoelii and P. vinckei). The isoelectric points (pI) of isozymes found in purified parasites were identical. SOD activities detected by isoelectrofocusing at pl 5.0, 5.6, and 6.4 were cyanide-sensitive and could be considered as having been adopted by the parasites from the host red blood cell. The three rodent malaria parasites also contained a cyanide-resistant, hydrogen peroxide-sensitive SOD activity not found in the host red blood cell. It is therefore concluded that the three rodent malaria parasites possess an endogenous SOD. Two bands of endogenous SOD were found at pl 6.2 and 6.8 for the three species, and one additional band was detected at pl 5.7 for P. berghei and P. vinckei. This first report in rodent Plasmodium of a cyanide-resistant, hydrogen peroxide-sensitive SOD suggests that these parasites may be capable of at least partly resisting activated oxygen species using an endogenous SOD.
对三种啮齿动物疟原虫（伯氏疟原虫、约氏疟原虫和文氏疟原虫）中的超氧化物歧化酶（SOD）进行了研究。在纯化的寄生虫中发现的同工酶的等电点（pI）相同。在pH 5.0、5.6和6.4处通过等电聚焦检测到的SOD活性对氰化物敏感，可认为是寄生虫从宿主红细胞中获得的。这三种啮齿动物疟原虫还含有一种宿主红细胞中未发现的抗氰化物、对过氧化氢敏感的SOD活性。因此得出结论，这三种啮齿动物疟原虫拥有内源性SOD。这三种疟原虫在pH 6.2和6.8处发现了两条内源性SOD条带，伯氏疟原虫和文氏疟原虫在pH 5.7处还检测到一条额外的条带。在啮齿动物疟原虫中首次报道的抗氰化物、对过氧化氢敏感的SOD表明，这些寄生虫可能能够利用内源性SOD至少部分抵抗活性氧。
