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通过聚合酶链反应区分旋毛虫分离株

Differentiation of Trichinella isolates by polymerase chain reaction.

作者信息

Soulé C, Guillou J P, Dupouy-Camet J, Vallet C, Pozio E

机构信息

C.N.E.V.A./Laboratoire Central de Recherche Vétérinaires, Maisons-Alfort, France.

出版信息

Parasitol Res. 1993;79(6):461-5. doi: 10.1007/BF00931583.

DOI:10.1007/BF00931583
PMID:8415558
Abstract

Oligonucleotide primers were synthesized for the polymerase-chain-reaction amplification of target DNA from two sequences of Trichinella spiralis. Six strains belonging to T. spiralis, T. nativa, T. britovi, T. pseudospiralis, and T. nelsoni were tested. Amplification products were obtained with T. spiralis, T. britovi, and T. nelsoni DNA from a 53-kDa antigen cDNA sequence and with T. spiralis and T. nelsoni DNA from a 1.6-kb repetitive DNA sequence. Differences in the length of the amplification products obtained from the repetitive sequence would enable a differentiation between T. spiralis and T. nelsoni, suggesting that the 1.6 kb repetitive DNA sequence would not be specific for T. spiralis. No amplification was detected for T. nativa or T. pseudospiralis DNA from the two sequences and for T. britovi DNA from the 1.6-kb repetitive DNA sequence.

摘要

合成了寡核苷酸引物，用于从旋毛虫的两个序列进行聚合酶链反应扩增目标DNA。测试了属于旋毛虫、本地旋毛虫、布氏旋毛虫、伪旋毛虫和纳氏旋毛虫的六个菌株。从53 kDa抗原cDNA序列的旋毛虫、布氏旋毛虫和纳氏旋毛虫DNA以及从1.6 kb重复DNA序列的旋毛虫和纳氏旋毛虫DNA中获得了扩增产物。从重复序列获得的扩增产物长度差异能够区分旋毛虫和纳氏旋毛虫，这表明1.6 kb重复DNA序列对旋毛虫不具有特异性。从这两个序列中未检测到本地旋毛虫或伪旋毛虫DNA的扩增，从1.6 kb重复DNA序列中未检测到布氏旋毛虫DNA的扩增。

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