Zhu G, McDougald L R
Department of Poultry Science, University of Georgia, Athens 30602.
Parasitol Res. 1993;79(6):480-4. doi: 10.1007/BF00931587.
Protein samples prepared from sporozoites of two ionophore-sensitive strains (WIS and Penn St) and three resistant strains (FS139, FS459, and FS462) of Eimeria tenella were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), native polyacrylamide gel electrophoresis (native PAGE), or two-dimensional electrophoresis with native PAGE and SDS-PAGE. Variant proteins that might be associated with ionophore resistance were observed in resistant field strains on native PAGE. When two-dimensional electrophoresis was conducted, four over-expressed peptides with approximate molecular weights of 97, 71, 70, and 50 kDa were observed in the FS139 field strain in comparison with the WIS laboratory strain.
从柔嫩艾美耳球虫的两个离子载体敏感株（WIS和宾夕法尼亚州立大学株）和三个抗性株（FS139、FS459和FS462）的子孢子制备的蛋白质样品，进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、非变性聚丙烯酰胺凝胶电泳（非变性PAGE）或非变性PAGE和SDS-PAGE的二维电泳。在非变性PAGE上，抗性田间株中观察到了可能与离子载体抗性相关的变异蛋白。进行二维电泳时，与WIS实验室株相比，在FS139田间株中观察到了四种分子量约为97、71、70和50 kDa的过表达肽。
