23S核糖体RNA中受tRNA保护的碱基U2555处的突变会影响翻译保真度。
Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity.
作者信息
O'Connor M, Dahlberg A E
机构信息
Section of Biochemistry, Brown University, Providence, RI 02912.
出版信息
Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8. doi: 10.1073/pnas.90.19.9214.
Abstract
A plasmid carrying a mutation in the highly conserved base U2555 in Escherichia coli 23S rRNA was isolated by selecting for suppression of the -1 frameshift mutation trpE91. U2555 is normally protected in chemical footprinting experiments by the aminoacyl residue of A-site-bound tRNA. Substitution of U2555 by adenine or guanine (but not by cytosine) increased readthrough of all three stop codons and +1 and -1 frameshifting. These effects on translational fidelity demonstrate the importance of U2555 for selection of the correct tRNA at the ribosomal A site.
摘要
通过筛选对-1移码突变trpE91的抑制作用，分离出了携带大肠杆菌23S rRNA中高度保守碱基U2555发生突变的质粒。在化学足迹实验中，U2555通常受到A位点结合的tRNA的氨酰基残基的保护。用腺嘌呤或鸟嘌呤（而非胞嘧啶）取代U2555，增加了所有三个终止密码子的通读以及+1和-1移码。这些对翻译保真度的影响证明了U2555对于在核糖体A位点选择正确tRNA的重要性。
相似文献
1
Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity.
Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8. doi: 10.1073/pnas.90.19.9214.
2
UGA suppression by a mutant RNA of the large ribosomal subunit.
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12309-13. doi: 10.1073/pnas.92.26.12309.
3
The involvement of two distinct regions of 23 S ribosomal RNA in tRNA selection.
J Mol Biol. 1995 Dec 15;254(5):838-47. doi: 10.1006/jmbi.1995.0659.
4
The influence of base identity and base pairing on the function of the alpha-sarcin loop of 23S rRNA.
Nucleic Acids Res. 1996 Jul 15;24(14):2701-5. doi: 10.1093/nar/24.14.2701.
5
Mutations in the peptidyl transferase region of E. coli 23S rRNA affecting translational accuracy.
Nucleic Acids Res. 1994 Feb 11;22(3):279-84. doi: 10.1093/nar/22.3.279.
6
Increased ribosomal accuracy increases a programmed translational frameshift in Escherichia coli.
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2315-9. doi: 10.1073/pnas.90.6.2315.
7
Base-pairing between 23S rRNA and tRNA in the ribosomal A site.
Mol Cell. 1999 Nov;4(5):859-64. doi: 10.1016/s1097-2765(00)80395-0.
8
A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome.
Nature. 1995 Sep 28;377(6547):309-14. doi: 10.1038/377309a0.
9
Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.
J Bacteriol. 1992 Dec;174(24):7896-901. doi: 10.1128/jb.174.24.7896-7901.1992.
10
The structure of helix 89 of 23S rRNA is important for peptidyl transferase function of Escherichia coli ribosome.
FEBS Lett. 2011 Oct 3;585(19):3073-8. doi: 10.1016/j.febslet.2011.08.030. Epub 2011 Aug 27.
引用本文的文献
1
Biological cost of aminoglycoside resistance Arm/Kam 16S rRNA methyltransferases from natural antibiotic producers and clinical pathogens.
Antimicrob Agents Chemother. 2025 Sep 3;69(9):e0074225. doi: 10.1128/aac.00742-25. Epub 2025 Jul 31.
2
Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome.
Nucleic Acids Res. 2023 Feb 28;51(4):1880-1894. doi: 10.1093/nar/gkac1273.
3
The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain.
iScience. 2022 Oct 20;25(11):105425. doi: 10.1016/j.isci.2022.105425. eCollection 2022 Nov 18.
4
Mutational characterization and mapping of the 70S ribosome active site.
Nucleic Acids Res. 2020 Mar 18;48(5):2777-2789. doi: 10.1093/nar/gkaa001.
5
Alterations in ribosomal protein L19 that decrease the fidelity of translation.
Biochimie. 2016 Sep-Oct;128-129:122-6. doi: 10.1016/j.biochi.2016.07.015. Epub 2016 Jul 28.
6
The presence of highly disruptive 16S rRNA mutations in clinical samples indicates a wider role for mutations of the mitochondrial ribosome in human disease.
Mitochondrion. 2015 Nov;25:17-27. doi: 10.1016/j.mito.2015.08.004. Epub 2015 Sep 5.
7
Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors.
RNA. 2015 Oct;21(10):1834-43. doi: 10.1261/rna.052613.115. Epub 2015 Aug 19.
8
Translational defects in a mutant deficient in YajL, the bacterial homolog of the parkinsonism-associated protein DJ-1.
J Bacteriol. 2010 Dec;192(23):6302-6. doi: 10.1128/JB.01077-10. Epub 2010 Oct 1.
9
Mutations at the accommodation gate of the ribosome impair RF2-dependent translation termination.
RNA. 2010 Sep;16(9):1848-53. doi: 10.1261/rna.2185710. Epub 2010 Jul 28.
10
RNA modifications: a mechanism that modulates gene expression.
Methods Mol Biol. 2010;629:1-19. doi: 10.1007/978-1-60761-657-3_1.
本文引用的文献
1
Codon-specific missense errors in vivo.
EMBO J. 1983;2(8):1351-6. doi: 10.1002/j.1460-2075.1983.tb01591.x.
2
The nucleotide sequence of the first externally suppressible--1 frameshift mutant, and of some nearby leaky frameshift mutants.
EMBO J. 1983;2(8):1345-50. doi: 10.1002/j.1460-2075.1983.tb01590.x.
3
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
J Mol Biol. 1981 May 15;148(2):107-27. doi: 10.1016/0022-2836(81)90508-8.
4
Increased translational fidelity caused by the antibiotic kasugamycin and ribosomal ambiguity in mutants harbouring the ksgA gene.
FEBS Lett. 1984 Nov 5;177(1):119-24. doi: 10.1016/0014-5793(84)80994-1.
5
Escherichia coli ribosomes translate in vivo with variable rate.
EMBO J. 1984 Dec 1;3(12):2895-8. doi: 10.1002/j.1460-2075.1984.tb02227.x.
6
Versatile low-copy-number plasmid vectors for cloning in Escherichia coli.
Gene. 1982 Jun;18(3):335-41. doi: 10.1016/0378-1119(82)90172-x.
7
Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reaction.
Arch Biochem Biophys. 1968 May;125(2):497-505. doi: 10.1016/0003-9861(68)90607-3.
8
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
9
10
A compilation of large subunit RNA sequences presented in a structural format.
Nucleic Acids Res. 1988;16 Suppl(Suppl):r175-269. doi: 10.1093/nar/16.suppl.r175.
Zotero 插件