Bryan D J, Miller R A, Costas P D, Wang K K, Seckel B R
Department of Plastic and Reconstructive Surgery, Lahey Clinic Medical Center, Burlington, Mass.
Plast Reconstr Surg. 1993 Oct;92(5):927-40.
The influence on nerve regeneration of the extracellular matrix glycoprotein laminin was studied after sciatic nerve transection in 90 outbred Sprague-Dawley rats. Nerve regeneration through basal lamina grafts was comparable with regeneration through traditional nerve grafts across gaps up to 2.0 cm in length. True axonal regeneration rather than axonal branching was demonstrated by retrograde horseradish peroxidase labeling of nerve cables. Pretreatment of basal lamina grafts with antilaminin antibodies reduced the total number of regenerated axons by 90 percent with a significant decrease of nerve conduction velocity and a significant impairment of walking track patterns. The basement membrane glycoprotein laminin serves a critical role in the regeneration of peripheral nerves through basal lamina grafts.
在90只远交群Sprague-Dawley大鼠坐骨神经横断后，研究了细胞外基质糖蛋白层粘连蛋白对神经再生的影响。通过基膜移植物的神经再生与通过传统神经移植物跨越长达2.0厘米间隙的再生相当。通过逆行辣根过氧化物酶标记神经束证明了真正的轴突再生而非轴突发支。用抗层粘连蛋白抗体预处理基膜移植物可使再生轴突总数减少90%，神经传导速度显著降低，行走轨迹模式明显受损。基底膜糖蛋白层粘连蛋白在通过基膜移植物的周围神经再生中起关键作用。
