Neto Humberto Santo, Sabha Mário José Jorge, Marques Maria Júlia
Departamento de Anatomia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil.
Cryobiology. 2005 Feb;50(1):112-5. doi: 10.1016/j.cryobiol.2004.10.005. Epub 2004 Dec 19.
The development of alternatives to nerve autografts for nerve repair remains a goal of surgeons. Muscle basal lamina grafts have a potential use as bioprostheses, but it is not known whether such grafts retain their ability to support axonal regeneration following storage. In this study, we examined the effect of cryopreservation on the ability of muscle basal lamina grafts to repair nerve lesions. Basal lamina grafts were prepared and cryopreserved for different times and at different temperatures. Their grafting potential was evaluated by examining axonal regeneration after autografting to lesions in rat sciatic nerves. Muscle basal lamina grafts cryopreserved for up to 30 weeks at -20 and -40 degrees C were successfully used. There were no significant differences in the parameters of axonal regeneration between cryopreserved and non-cryopreserved grafts. In conclusion, muscle basal lamina autografts retain their potential usefulness for nerve repair after cryopreservation, providing a basis for the development of a bioprostheses from muscle basal lamina.
开发用于神经修复的神经自体移植替代物仍是外科医生的一个目标。肌肉基膜移植物有作为生物假体的潜在用途，但尚不清楚此类移植物在储存后是否仍保留支持轴突再生的能力。在本研究中，我们检测了冷冻保存对肌肉基膜移植物修复神经损伤能力的影响。制备肌肉基膜移植物并在不同时间和不同温度下进行冷冻保存。通过检查自体移植到大鼠坐骨神经损伤处后的轴突再生情况来评估它们的移植潜力。在-20℃和-40℃下冷冻保存长达30周的肌肉基膜移植物成功得到使用。冷冻保存的移植物和未冷冻保存的移植物在轴突再生参数方面没有显著差异。总之，肌肉基膜自体移植物在冷冻保存后仍保留其对神经修复的潜在用途，为开发基于肌肉基膜的生物假体提供了依据。
