Tutino M L, Scarano G, Marino G, Sannia G, Cubellis M V
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
J Bacteriol. 1993 Jan;175(1):299-302. doi: 10.1128/jb.175.1.299-302.1993.
A DNA fragment containing the trpEGC gene cluster was isolated from the thermoacidophilic archaebacterium Sulfolobus solfataricus. The products of trpE, trpG, and trpC from S. solfataricus were compared to the homologous products from a eukaryote, a eubacterium, and two archaebacteria, namely, a methanogen and an extreme halophile. They appeared to be equally related to the proteins from Escherichia coli and Saccharomyces cerevisiae, the percentages of conserved amino acids being roughly the same as those measured when comparing the eubacterial and eukaryotic sequences directly. These percentages did not rise significantly when a comparison with the proteins from Haloferax volcanii was drawn, while a slightly closer relationship with the proteins from Methanococcus thermoautotrophicum was found.
从嗜热嗜酸古细菌嗜热栖热菌中分离出一个包含色氨酸EGC基因簇的DNA片段。将嗜热栖热菌的色氨酸E、色氨酸G和色氨酸C产物与来自真核生物、真细菌以及两种古细菌（即产甲烷菌和嗜盐嗜碱菌）的同源产物进行了比较。它们与大肠杆菌和酿酒酵母的蛋白质的亲缘关系似乎相同，保守氨基酸的百分比与直接比较真细菌和真核生物序列时测得的百分比大致相同。与沃氏嗜盐菌的蛋白质进行比较时，这些百分比没有显著上升，而发现与嗜热自养甲烷球菌的蛋白质关系稍近。
