Bories P N, Antoniotti C, Pons J L, Leluan G
Laboratoire Central, Hôpital Albert Chenevier, Créteil, France.
J Clin Microbiol. 1993 Jan;31(1):157-9. doi: 10.1128/jcm.31.1.157-159.1993.
Esterase electrophoresis was used to study 10 strains of Clostridium argentinense, including 7 toxigenic and 3 nontoxigenic strains. On the basis of the electrophoretic mobilities and hydrolytic specificities toward five synthetic substrates, different esterase profiles could be defined for almost all strains, revealing the heterogeneity of bacterial clones. Therefore, electrophoretic polymorphism of esterases can be used for differentiation of C. argentinense in population genetic or epidemiological studies.
酯酶电泳被用于研究10株阿根廷梭菌,其中包括7株产毒菌株和3株不产毒菌株。基于电泳迁移率以及对五种合成底物的水解特异性,几乎所有菌株都可定义出不同的酯酶图谱,这揭示了细菌克隆的异质性。因此,酯酶的电泳多态性可用于群体遗传学或流行病学研究中阿根廷梭菌的鉴别。