Legay C, Bon S, Massoulié J
Laboratoire de Neurobiologie, CNRS UA 295, Ecole Normale Supérieure, Paris, France.
FEBS Lett. 1993 Jan 4;315(2):163-6. doi: 10.1016/0014-5793(93)81155-s.
We amplified by PCR and characterized a fragment of cDNA from rat spleen, encoding the distinctive C-terminal region of the acetylcholinesterase (AChE) H subunit. A recombinant vector encoding this subunit was constructed and expressed in COS cells: the H subunits produced glycophosphatidylinositol (GPI)-anchored dimers, showing that the spleen cDNA fragment contained a functional GPI cleavage/attachment site. Using PCR, we did not detect mRNAs encoding AChE H in rat muscle or hypothalamus. In the liver of 16-day rat embryos, we found both H and T transcripts, in agreement with the presence of both GPI-anchored dimers and amphiphilic monomers of type II. In addition, we detected 'read-through' (R) transcripts, in which regular introns are spliced, but the intervening sequence between the common exon 4 and the alternative exon 5 (H) is maintained.
我们通过聚合酶链反应（PCR）扩增并鉴定了大鼠脾脏的一段互补脱氧核糖核酸（cDNA）片段，该片段编码乙酰胆碱酯酶（AChE）H亚基独特的C末端区域。构建了编码该亚基的重组载体并在COS细胞中表达：产生的H亚基为糖基磷脂酰肌醇（GPI）锚定二聚体，表明脾脏cDNA片段含有功能性GPI切割/附着位点。使用PCR，我们在大鼠肌肉或下丘脑未检测到编码AChE H的信使核糖核酸（mRNA）。在16日龄大鼠胚胎的肝脏中，我们发现了H和T转录本，这与II型GPI锚定二聚体和两亲性单体的存在一致。此外，我们检测到“通读”（R）转录本，其中常规内含子被剪接，但常见外显子4和可变外显子5（H）之间的间隔序列得以保留。
