Orme I M, Furney S K, Skinner P S, Roberts A D, Brennan P J, Russell D G, Shiratsuchi H, Ellner J J, Weiser W Y
Department of Microbiology, Colorado State University, Fort Collins 80523.
Infect Immun. 1993 Jan;61(1):338-42. doi: 10.1128/iai.61.1.338-342.1993.
Infections caused by Mycobacterium avium, the most common form of diseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12-kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.
鸟分枝杆菌引起的感染是艾滋病患者中最常见的播散性细菌疾病形式，由于其对多种抗分枝杆菌药物具有耐药性，因此难以治疗。本研究结果表明，重组迁移抑制因子是一种12 kDa的分子，最近通过对人T细胞杂交瘤的cDNA进行COS-1细胞表达筛选分离得到，它对一组鸟分枝杆菌临床分离株在骨髓来源的小鼠巨噬细胞和培养的人血单核细胞中的生长具有强大的抑制活性。在重组迁移抑制因子中培养的这些细胞表现出各种激活迹象，包括细胞分裂、形态变化，如大量吞噬溶酶体融合的证据，以及肿瘤坏死因子分泌增加。
