Thurman G B, Braude I A, Gray P W, Oldham R K, Stevenson H C
J Immunol. 1985 Jan;134(1):305-9.
By utilizing elutriation-purified human monocytes, we found that human interferon (IFN) inhibits monocyte migration in a manner similar to migration inhibitory factor (MIF) and does it without demonstrable cytotoxicity. We observed that human IFN-gamma is 10 to 300 times more potent in its MIF activity than is IFN-alpha and that monoclonal antibodies (MoAb) can be used to distinguish between them. Studies with recombinant IFN-gamma indicate that the migration inhibition seen with natural IFN-gamma is due to IFN-gamma itself and is not due to co-purification of another lymphokine with the natural IFN-gamma. Although interferons exhibit MIF activities, there are apparently other cytokines, without antiviral activity, that also have MIF activities. MIF from the lymphoblastoid cell line RPMI 1788 was not neutralized by MoAb to IFN. However, MIF activity in supernatant fluid from human peripheral blood lymphocyte cultures stimulated with Con A-Sepharose was completely neutralized with MoAb anti-IFN-gamma. These data indicate that MIF is really a family of cytokines that inhibit macrophage/monocyte migration and that the major portion of MIF activity associated with crude supernatant of mitogen-stimulated lymphocytes is due to IFN-gamma.
通过利用淘析纯化的人单核细胞，我们发现人干扰素（IFN）以类似于迁移抑制因子（MIF）的方式抑制单核细胞迁移，并且在无明显细胞毒性的情况下做到这一点。我们观察到，人γ干扰素在其MIF活性方面比α干扰素强10至300倍，并且单克隆抗体（MoAb）可用于区分它们。对重组γ干扰素的研究表明，天然γ干扰素所见的迁移抑制是由于γ干扰素本身，而不是由于另一种淋巴因子与天然γ干扰素共纯化所致。尽管干扰素表现出MIF活性，但显然还有其他无抗病毒活性的细胞因子也具有MIF活性。来自淋巴母细胞系RPMI 1788的MIF不能被抗IFN的MoAb中和。然而，用刀豆蛋白A - 琼脂糖刺激的人外周血淋巴细胞培养上清液中的MIF活性可被抗γ干扰素的MoAb完全中和。这些数据表明，MIF实际上是一类抑制巨噬细胞/单核细胞迁移的细胞因子，并且与丝裂原刺激的淋巴细胞粗上清液相关的MIF活性的主要部分是由于γ干扰素。
