McClenaghan M, Herring A J, Aitken I D
Infect Immun. 1984 Aug;45(2):384-9. doi: 10.1128/iai.45.2.384-389.1984.
Preparations of DNA from 12 Chlamydia psittaci isolates and one Chlamydia trachomatis strain were compared by restriction endonuclease analysis. Polyacrylamide gel electrophoresis, followed by silver staining, resulted in optimal resolution of fragments generated by digestion. By this technique, four distinct electropherotypes were demonstrated when ovine abortion, ovine arthritis, and avian and Cal10 strains of C. psittaci were examined. Minor profile differences allowed the discrimination of avian isolates derived from psittacine and columbiforme species, and the Cal10 DNA electropherotype was shown to have features in common with these profiles. However, there were no detectable differences in the DNA patterns of eight ovine abortion isolates.
通过限制性内切酶分析比较了12株鹦鹉热衣原体分离株和1株沙眼衣原体菌株的DNA制备物。采用聚丙烯酰胺凝胶电泳，随后进行银染，可实现消化产生的片段的最佳分辨率。通过该技术，在检查绵羊流产、绵羊关节炎以及鹦鹉热衣原体的禽类和Cal10菌株时，发现了四种不同的电泳型。微小的图谱差异使得源自鹦鹉科和鸽形目物种的禽类分离株得以区分，并且Cal10 DNA电泳型显示出与这些图谱有共同特征。然而，8株绵羊流产分离株的DNA图谱没有可检测到的差异。
