Paz P, Sánchez A, Melcón C, Fernández J G, Chamorro C A
Departamento Biología Celular y Anatomía, Facultad de Veterinaria, Universidad de León, Spain.
Anat Rec. 1993 Feb;235(2):296-302. doi: 10.1002/ar.1092350213.
The process of T-lymphocyte differentiation within the thymus involves a series of molecular interactions. In this work we have carried out an analysis of the chick thymus microenvironment in order to evaluate its heterogeneity during development. We have produced 11 monoclonal antibodies whose staining patterns detected by the immunoperoxidase technique allowed us to divide them into five groups. A first group (E19-E2, P0-E5, and P15-T1) binds to thymic medullary stroma showing a reticular pattern on medullary epithelial cells and whose significance would be related to thymic stromal secretion. The second group of monoclonal antibodies (P15-T3) stains thymic corpuscles of 10- and 15-day chicks. The third group of antibodies includes P0-E1, P0-E3, P5-A6, and P15-T2 whose staining pattern is both medullary and cortical. The fourth group (P10-HB1 and P10-HB2) binds to thymic stromal and cortical thymocytes, and the fifth group (P5-A1) is characterized by the staining of medullary vessels of 5-day chicks. These five groups of monoclonal antibodies corroborate the existence of an antigenic diversity of the chick thymus microenvironment. Their possible relationships with T-cell differentiation and stromal-thymocyte interactions are discussed.
胸腺内T淋巴细胞的分化过程涉及一系列分子相互作用。在这项工作中，我们对鸡胸腺微环境进行了分析，以评估其在发育过程中的异质性。我们制备了11种单克隆抗体，通过免疫过氧化物酶技术检测到的染色模式使我们能够将它们分为五组。第一组（E19 - E2、P0 - E5和P15 - T1）与胸腺髓质基质结合，在髓质上皮细胞上呈现网状模式，其意义可能与胸腺基质分泌有关。第二组单克隆抗体（P15 - T3）对10日龄和15日龄雏鸡的胸腺小体进行染色。第三组抗体包括P0 - E1、P0 - E3、P5 - A6和P15 - T2，其染色模式同时出现在髓质和皮质。第四组（P10 - HB1和P10 - HB2）与胸腺基质和皮质胸腺细胞结合，第五组（P5 - A1）的特征是对5日龄雏鸡的髓质血管进行染色。这五组单克隆抗体证实了鸡胸腺微环境存在抗原多样性。文中讨论了它们与T细胞分化以及基质 - 胸腺细胞相互作用的可能关系。
