Reyes R, Sánchez-Vázquez M L, Delgado N M
Unidad de Investigación Biomédica de Oriente, Centro Médico Nacional, General de Division Manuel Avila Camacho, Instituto Mexicano del Seguro Social, Puebla.
Arch Androl. 1993 Jan-Feb;30(1):73-7. doi: 10.3109/01485019308988372.
Glycolitic metabolism under basal conditions and its modifications by the combined action of heparin and GSH were studied in human sperm. Respirometric data indicated that the amount of U. L. [14C]-glucose converted to 14CO2 increased with the incubation time, being almost linear for up to 60 min and then leveling off at 120 and 150 min (594 and 620 nmol of [14C]-glucose/10(8) spermatozoa, respectively). When spermatozoa were incubated in the presence of heparin-GSH such behavior completely changed, showing a decrease (approximately 50%) in glucose metabolism with values of 254 and 366 nmol of [14C]-glucose/10(8) spermatozoa at the same incubation times as the basal consumption. When these results were compared with the kinetic of the swollen nuclei it was seen that at 30 min 44% of the spermatozoa have its nuclei swollen with a glucose uptake value of 91 nmol/10(8) spermatozoa, and at 150 min when nearly all the spermatozoa nuclei are swollen (95%) the glucose uptake increases fourfold more than the initial rate at 30 min. Therefore, it is possible to suggest the existence of an energy contribution by the sperm to the male pronuclei formation mechanism.
在人类精子中研究了基础条件下的糖酵解代谢及其受肝素和谷胱甘肽联合作用的影响。呼吸测定数据表明，转化为(^{14}CO_2)的(U.L.\ [^{14}C])-葡萄糖量随孵育时间增加，在长达60分钟时几乎呈线性增加，然后在120和150分钟时趋于平稳（分别为(594)和(620)纳摩尔([^{14}C])-葡萄糖/(10^8)个精子）。当精子在肝素 - 谷胱甘肽存在下孵育时，这种行为完全改变，葡萄糖代谢降低（约50%），在与基础消耗相同的孵育时间下，([^{14}C])-葡萄糖值分别为(254)和(366)纳摩尔/(10^8)个精子。当将这些结果与肿胀细胞核的动力学进行比较时发现，在30分钟时，44%的精子细胞核肿胀，葡萄糖摄取值为(91)纳摩尔/(10^8)个精子，而在150分钟时，几乎所有精子细胞核都肿胀（95%），葡萄糖摄取比30分钟时的初始速率增加了四倍。因此，可以推测精子对雄原核形成机制存在能量贡献。
