Emi N, Kitaori K, Seto M, Ueda R, Saito H, Takahashi T
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Immunogenetics. 1993;37(3):193-8. doi: 10.1007/BF00191884.
A novel cDNA clone, A15, was isolated by the differential screening of a cDNA library of an immature T cell line, HPB-ALL using radioactive cDNA probes from the mRNA of either HPB-ALL or peripheral blood lymphocytes. It hybridized to a single mRNA species of about 2.0 kilobases which is expressed in HPB-ALL cell line, but not in the PBL or a promyelocytic leukemia cell line, HL-60. The A15 gene codes for a protein of 244 amino acids which contains four potential transmembrane domains and four possible N-linked glycosylation sites. A computer-aided comparison showed a marked similarity to several other membrane proteins: CD9, CD37, CD53, TAPA-1, Sm23, CO-029, and ME491/CD63.
通过用来自HPB - ALL或外周血淋巴细胞mRNA的放射性cDNA探针，对未成熟T细胞系HPB - ALL的cDNA文库进行差异筛选，分离出一个新的cDNA克隆A15。它与一种约2.0千碱基的单一mRNA物种杂交，该mRNA在HPB - ALL细胞系中表达，但在PBL或早幼粒细胞白血病细胞系HL - 60中不表达。A15基因编码一种由244个氨基酸组成的蛋白质，该蛋白质含有四个潜在的跨膜结构域和四个可能的N - 连接糖基化位点。计算机辅助比较显示它与其他几种膜蛋白有显著相似性：CD9、CD37、CD53、TAPA - 1、Sm23、CO - 029和ME491/CD63。
