Gomolka M, Epplen C, Buitkamp J, Epplen J T
Molekulare Humangenetik, Ruhr-Universität, Bochum, Germany.
Immunogenetics. 1993;37(4):257-65. doi: 10.1007/BF00187451.
The human T-cell receptor (Tcr) Vb6 family has been scrutinized for polymorphisms, both in coding as well as in intronic sequences by polymerase chain reaction (PCR), subsequent multiple electroblot hybridizations, and sequence analysis. Multiplex PCR is an efficient means of screening for Tcr variability. Four novel loci could be distinguished and several new alleles are described including two pseudogenes. The Vb6 family is characterized by an intronic stretch of simple repetitive (gt)n sequences. These elements are hypervariable, especially in the Vb6.7 subfamily, where they are particularly long. The unexpected persistence of simple repetitive sequences in Tcr and major histocompatibility complex (MHC) class II genes over extended periods of the vertebrate evolutionary history can be interpreted in parallel terms in both gene families.
通过聚合酶链反应（PCR）、随后的多次电印迹杂交和序列分析，对人类T细胞受体（Tcr）Vb6家族的编码序列和内含子序列中的多态性进行了仔细研究。多重PCR是筛选Tcr变异性的有效方法。可以区分出四个新位点，并描述了几个新等位基因，包括两个假基因。Vb6家族的特征是内含子中有一段简单重复的（gt）n序列。这些元件具有高度变异性，特别是在Vb6.7亚家族中，它们特别长。在脊椎动物进化历史的很长一段时间里，Tcr和主要组织相容性复合体（MHC）II类基因中简单重复序列的意外持续存在，可以在这两个基因家族中并行解释。
