Levenson C W, Shay N F, Lee-Ambrose L M, Cousins R J
Department of Food Science and Human Nutrition, University of Florida, Gainesville 32611.
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):712-5. doi: 10.1073/pnas.90.2.712.
The cysteine-rich intestinal protein (CRIP) is an intestinal zinc-binding protein containing a single copy of a cysteine-rich domain known as the LIM motif. CRIP mRNA and protein levels increased in the rat small intestine throughout the suckling period, reaching highest levels by the late weanling stage. A similar developmental pattern of CRIP protein levels was also detected by an increase in zinc binding to CRIP-containing HPLC fractions of intestinal cytosol. Administration of the synthetic glucocorticoid hormone dexamethasone to neonates caused the precocious rise of CRIP mRNA and protein. In adult rats, CRIP mRNA levels were not significantly altered by dexamethasone. Maximal CRIP mRNA content was detected in cells from the mid-villus, as confirmed by expression of cryptdin mRNA. In this report we show the glucocorticoid regulation of the LIM motif-containing protein CRIP and suggest that glucocorticoid hormones play a role in developmental regulation of CRIP.
富含半胱氨酸的肠道蛋白（CRIP）是一种肠道锌结合蛋白，含有一个称为LIM基序的富含半胱氨酸结构域的单拷贝。在整个哺乳期，大鼠小肠中的CRIP mRNA和蛋白质水平升高，在断奶后期达到最高水平。通过与肠道细胞质中含CRIP的HPLC级分的锌结合增加，也检测到CRIP蛋白水平的类似发育模式。向新生儿施用合成糖皮质激素地塞米松导致CRIP mRNA和蛋白质早熟升高。在成年大鼠中，地塞米松未显著改变CRIP mRNA水平。如隐窝蛋白mRNA的表达所证实，在绒毛中部的细胞中检测到最大的CRIP mRNA含量。在本报告中，我们展示了含LIM基序蛋白CRIP的糖皮质激素调节，并表明糖皮质激素在CRIP的发育调节中起作用。
