Araki S, Ishida T, Yamamoto T, Kaji K, Hayashi H
Sugashima Marine Biological Laboratory, School of Science, Nagoya University, Mie, Japan.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):148-53. doi: 10.1006/bbrc.1993.1023.
Vascular degeneration appears to play crucial roles in producing many vascular malfunctions (1-3). In order to identify specific inducers of programmed death in vascular endothelial cells (VEC), examinations were made of the effects of substances that are known to affect the vascular system by using VEC in culture (4,5). We found that hemorrhagic snake venoms induced apoptotic cell death or programmed cell death of VEC. By contrast, neurotoxic snake venoms did not induce programmed cell death but caused necrosis at much higher doses of the venoms. No effect of hemorrhagic venom was observed with many types of cultured cells other than VEC. Thus, hemorrhagic snake venom appears to be a useful tool for studies of the molecular mechanisms of vascular apoptosis. The results also suggest a possible mechanism of action of hemorrhagic snake venom on the vascular system.
血管变性似乎在引发多种血管功能障碍中起着关键作用(1 - 3)。为了确定血管内皮细胞（VEC）中程序性死亡的特定诱导因素，我们利用培养的VEC研究了已知会影响血管系统的物质的作用(4,5)。我们发现出血性蛇毒可诱导VEC发生凋亡性细胞死亡或程序性细胞死亡。相比之下，神经毒性蛇毒不会诱导程序性细胞死亡，但在高得多的毒液剂量下会导致坏死。除VEC外，在许多类型的培养细胞中未观察到出血性毒液的作用。因此，出血性蛇毒似乎是研究血管凋亡分子机制的有用工具。这些结果还提示了出血性蛇毒对血管系统的一种可能作用机制。
