Avanzini G, Vergnes M, Spreafico R, Marescaux C
Department of Neurophysiology, Istituto Neurologico C. Besta, Milan, Italy.
Epilepsia. 1993 Jan-Feb;34(1):1-7. doi: 10.1111/j.1528-1157.1993.tb02369.x.
The pathophysiologic role of the reticular thalamic nucleus (RT) in rat generalized nonconvulsive epilepsy was investigated in the selected strain GAERS (genetic absence epilepsy rats from Strasbourg). After the RT was lesioned by the excitotoxic agent ibotenic acid stereotaxically injected in previously callosotomized rats, a disruption of ipsilateral spike and wave discharges (SWD) was observed in freely moving animals. In a second group of animals Cd2+ (0.5-1.5 microliter, 1 mM), which is known to block Ca2+ and Ca(2+)-dependent K+ conductances (gK+ (Ca2+)), was injected into the thalamus. Cd2+ reversibly suppressed ipsilateral SWD when injected in RT, whereas it slightly reduced SWD expression when injected in the ventrobasal (VB) complex. The difference was highly significant. We conclude that Ca(2+)-dependent oscillatory properties of the RT are critical for expression of genetically determined SWD in GAERS.
在选定的GAERS品系（来自斯特拉斯堡的遗传性失神癫痫大鼠）中，研究了丘脑网状核（RT）在大鼠全身性非惊厥性癫痫中的病理生理作用。在先前已进行胼胝体切开术的大鼠中，通过立体定向注射兴奋性毒性剂鹅膏蕈氨酸对RT进行损伤后，在自由活动的动物中观察到同侧棘波和慢波放电（SWD）受到破坏。在第二组动物中，将已知可阻断Ca2+和Ca(2+)依赖性K+电导（gK+(Ca2+)）的Cd2+（0.5 - 1.5微升，1 mM）注入丘脑。当注入RT时，Cd2+可逆地抑制同侧SWD，而当注入腹侧基底（VB）复合体时，它仅轻微降低SWD的表达。差异非常显著。我们得出结论，RT的Ca(2+)依赖性振荡特性对于GAERS中遗传决定的SWD的表达至关重要。
