Russo P, Gingras D, Bendayan M
Département de Pathologie, Hôpital Ste-Justine, Montréal, Québec, Canada.
Am J Pathol. 1993 Jan;142(1):261-71.
Anionic sites play a key role in the charge selectivity of glomerular filtration as well as in the maintenance of the structural integrity of the visceral epithelium and podocytes. Alterations in these sites are believed to be a major factor underlying human idiopathic nephrosis and puromycin-nephrosis in the rat. The poly-L-lysine-gold complex was used for the ultrastructural detection of anionic sites in renal glomeruli of patients with idiopathic nephrosis as well as of rats with puromycin-induced nephrosis, allowing for study of the changes occurring in the anionic sites during nephrosis and for the comparison between human disease and this experimental model. In both normal human and rat controls, the probe was detected on epithelial and endothelial cell surfaces and on the glomerular basement membrane, mainly in both laminae rarae. In proteinuric rats, a decrease in labeling intensity was noted on podocyte membranes and in the lamina rara externa, with a corresponding increase in the more central areas of the glomerular basement membrane. These changes were not as evident in proteinuric humans. Furthermore, a reduction in labeling density was noted in the glomerular basement membrane of proteinuric animals, although this could not be substantiated in human tissues. Poly-L-lysine-gold is a useful probe for anionic sites in fixed tissues, and allows for comparison between human disease and its experimental counterpart.
阴离子位点在肾小球滤过的电荷选择性以及脏层上皮和足细胞结构完整性的维持中起关键作用。这些位点的改变被认为是人类特发性肾病和大鼠嘌呤霉素肾病的主要潜在因素。聚-L-赖氨酸-金复合物用于特发性肾病患者以及嘌呤霉素诱导肾病大鼠的肾小球中阴离子位点的超微结构检测，从而能够研究肾病期间阴离子位点发生的变化，并对人类疾病与该实验模型进行比较。在正常人类和大鼠对照中，探针主要在两个透明层中检测到上皮和内皮细胞表面以及肾小球基底膜上。在蛋白尿大鼠中，足细胞膜和外透明层的标记强度降低，而肾小球基底膜更中央区域的标记强度相应增加。这些变化在蛋白尿人类中不那么明显。此外，蛋白尿动物的肾小球基底膜标记密度降低，尽管在人体组织中无法证实这一点。聚-L-赖氨酸-金是固定组织中阴离子位点的有用探针，并允许对人类疾病与其实验对应物进行比较。
