Palmgren M G, Christensen G
August Krogh Institute, University of Copenhagen, Denmark.
FEBS Lett. 1993 Feb 15;317(3):216-22. doi: 10.1016/0014-5793(93)81279-9.
In plants and fungi, the transport of solutes across the plasma membrane (pm) is driven by a proton pump (H(+)-ATPase) that produces an electric potential and a pH gradient. We expressed AHA2, a member of the Arabidopsis thaliana pm H(+)-ATPase gene family, in yeast cells in which transcription of the endogenous pm H(+)-ATPase gene (pma1) had been turned off. AHA2 was expressed mainly in intracellular membranes and only supported very slow growth of transformed yeast cells. Removal of the last 92 C-terminal amino acids from the plant H(+)-ATPase produced an enzyme with 2-3-fold higher specific ATPase activity than the wild-type plant enzyme. Surprisingly, the truncated H(+)-ATPase was now targetted to the yeast pm and fully supported normal yeast growth.
在植物和真菌中，溶质跨质膜（pm）的运输由质子泵（H(+)-ATP酶）驱动，该质子泵产生电势和pH梯度。我们在已关闭内源性质膜H(+)-ATP酶基因（pma1）转录的酵母细胞中表达了拟南芥质膜H(+)-ATP酶基因家族的成员AHA2。AHA2主要在内膜中表达，仅支持转化酵母细胞非常缓慢的生长。从植物H(+)-ATP酶中去除最后92个C末端氨基酸后产生的一种酶，其比野生型植物酶具有高2至3倍的比ATP酶活性。令人惊讶的是，截短的H(+)-ATP酶现在靶向酵母质膜，并完全支持酵母正常生长。
