Hindré F, Le Plouzennec M, de Certaines J D, Foultier M T, Patrice T, Simonneaux G
Laboratoire de Chimie Organométallique, Faculté des Sciences, Université de Rennes, France.
J Magn Reson Imaging. 1993 Jan-Feb;3(1):59-65. doi: 10.1002/jmri.1880030111.
Tetra-p-aminophenylporphyrin (TPP) was conjugated with gadolinium diethylenetriaminepentaacetic acid (DTPA) and used as a contrast agent in magnetic resonance (MR) imaging to achieve tumor selectivity in nude mice. A substantial decrease in T1 was measured in excised tissues (kidneys, tumor, and liver) from mice that received the porphyrin derivative Gd2(DTPA)4 TPP. Toxicity and phototoxicity were less than those obtained with hematoporphyrin derivative in both L1210 lymphoblastic leukemia cells and HT 29 human colonic cancer cells, as determined with in vitro assays. MR images showed an enhancement of contrast between the tumor and adjacent tissue after injection of this agent. The results indicate that Gd2(DTPA)4TPP could be a useful prototype paramagnetic porphyrin MR imaging contrast agent with an affinity for tumors.
四对氨基苯基卟啉（TPP）与钆二乙烯三胺五乙酸（DTPA）偶联，并用作磁共振（MR）成像中的造影剂，以在裸鼠中实现肿瘤选择性。在接受卟啉衍生物Gd2（DTPA）4 TPP的小鼠的切除组织（肾脏、肿瘤和肝脏）中测量到T1有显著下降。体外试验表明，在L1210淋巴细胞白血病细胞和HT 29人结肠癌细胞中，其毒性和光毒性均低于血卟啉衍生物。MR图像显示注射该造影剂后肿瘤与相邻组织之间的对比度增强。结果表明，Gd2（DTPA）4TPP可能是一种对肿瘤具有亲和力的有用的顺磁性卟啉MR成像造影剂原型。
